B6.129-St3gal2^tm2Jxm/J

Stock number: 006898
Product name: ST3Gal-II^F
Research areas: Research Tools

Description

These floxed mutant mice possess loxP sites flanking exon containing the large sialyl motif of the St3gal2 gene. This strain may be useful for generating conditional mutations in applications related to epilepsy and inflammation.

Detailed description

Mice homozygous for the floxed targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele.

Development

A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette in the intron downstream of the exon containing the large sialyl motif and a loxP site in the upstream intron. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl ⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with a Cre expression plasmid to excise the neo fragment and leave the floxed exon. Gancyclovir-resistant ES cells were injected into blastocysts. Chimeric animals were backcrossed to C57BL/6 at least 9 times by the donating laboratory.

Allele

Symbol: St3gal2^tm2Jxm
Name: targeted mutation 2, Jamey Marth
MGI accession ID: MGI:3699715
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: St3gal2
Marker name: ST3 beta-galactoside alpha-2,3-sialyltransferase 2
Chromosome: 8
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of the exon containing the large sialyl motif and a loxP site upstream of the exon. Correctly targeted ES cells were transfected with a Cre expression plasmid to excise the neo fragment, leaving the floxed exon.