These floxed mutant mice possess loxP sites flanking exon containing the large sialyl motif of the St3gal2 gene. This strain may be useful for generating conditional mutations in applications related to epilepsy and inflammation.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
Mice homozygous for the floxed targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele.
A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette in the intron downstream of the exon containing the large sialyl motif and a loxP site in the upstream intron. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl +-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with a Cre expression plasmid to excise the neo fragment and leave the floxed exon. Gancyclovir-resistant ES cells were injected into blastocysts. Chimeric animals were backcrossed to C57BL/6 at least 9 times by the donating laboratory.
|Allele Name||targeted mutation 2, Jamey Marth|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||St3gal2, ST3 beta-galactoside alpha-2,3-sialyltransferase 2|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of the exon containing the large sialyl motif and a loxP site upstream of the exon. Correctly targeted ES cells were transfected with a Cre expression plasmid to excise the neo fragment, leaving the floxed exon.|
|Mutations Made By|| |
Jamey Marth, Burnham Inst at Univ Calif Santa Barbara
When maintained as a live colony, heterozygous x C57BL/6 crosses are typically used.
When using the ST3Gal-IIF mouse strain in a publication, please cite the originating article(s) and include JAX stock #006898 in your Materials and Methods section.