B6.129-St3gal1^tm2Jxm/J

Stock number: 006897
Product name: ST3Gal-I^F
Research areas: Research Tools

Description

These floxed mutant mice possess loxP sites flanking exon 2 of the St3gal1 gene. This strain may be useful for generating conditional mutations in applications related to epilepsy and inflammation.

Detailed description

Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele.

Development

A targeting vector was used to place a loxP site in the intron downstream of exon 2 and a floxed neomycin-thymidine kinase expression cassette in the upstream intron. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl ⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with Cre expression plasmid to excise the neomycin-thymidine cassette, leaving a floxed exon 2. Gancyclovir-resistant ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 ten times by the donating laboratory.

Allele

Symbol: St3gal1^tm2Jxm
Name: targeted mutation 2, Jamey Marth
MGI accession ID: MGI:3699717
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: St3gal1
Marker name: ST3 beta-galactoside alpha-2,3-sialyltransferase 1
Chromosome: 15
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette upstream of exon two and an additional loxP site in intron 2. ES cells were transfected with Cre expression plasmid to excise the neo fragment, leaving the floxed gene.