These floxed mutant mice possess loxP sites flanking exon 2 of the St3gal1 gene. This strain may be useful for generating conditional mutations in applications related to epilepsy and inflammation.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele.
A targeting vector was used to place a loxP site in the intron downstream of exon 2 and a floxed neomycin-thymidine kinase expression cassette in the upstream intron. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl +-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with Cre expression plasmid to excise the neomycin-thymidine cassette, leaving a floxed exon 2. Gancyclovir-resistant ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 ten times by the donating laboratory.
|Allele Name||targeted mutation 2, Jamey Marth|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||St3gal1, ST3 beta-galactoside alpha-2,3-sialyltransferase 1|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette upstream of exon two and an additional loxP site in intron 2. ES cells were transfected with Cre expression plasmid to excise the neo fragment, leaving the floxed gene.|
|Mutations Made By|| |
Jamey Marth, Burnham Inst at Univ Calif Santa Barbara
When maintained as a live colony, homozygous crosses may be used.
When using the ST3Gal-IF mouse strain in a publication, please cite the originating article(s) and include JAX stock #006897 in your Materials and Methods section.