Mice homozygous for the Mgat3 knock-out mutation may be useful in studies of tumor progression.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. The mice exhibit normal developmental and physiologic parameters. No alterations are apparent in the neural system, circulating leukocytes, erythrocytes or in serum metabolite levels that reflect kidney function.
A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette upstream of the single protein-coding sequence exon of the gene and an additional loxP site downstream of the exon. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with a Cre expression plasmid to excise the neo fragment. Gancyclovir-resistant ES cells were injected into blastocysts. Chimeric animals were crossed with ZP3-Cre transgenic mates to produce the deleted Type 1 allele. This strain was backcrossed to C57BL/6 inbred mates at least nine times by the donating laboratory.
|Allele Name||targeted mutation 1, Jamey Marth|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Mgat3, mannoside acetylglucosaminyltransferase 3|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A construct was created in which loxP sites flanked the protein-encoding exon of the gene. After Cre recombinase treatment, the protein-encoding exon of the gene was excised, resulting in a null allele.|
|Mutations Made By|| |
Jamey Marth, Burnham Inst at Univ Calif Santa Barbara
When maintained as a live colony, heterozygous or homozygous crosses may be used.
When using the Mgat3 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006893 in your Materials and Methods section.