The CD19-Cre knock-in/knock-out allele has a Cre recombinase gene inserted into the first coding exon of the CD19 antigen gene; both abolishing endogenous Cd19 gene function and placing cre expression under the control of the endogenous Cd19 promoter/enhancer elements. Cre recombinase expression is directed at the earliest stages and throughout B-lymphocyte development and differentiation. Homozygous mice are also useful for studying B cell-deficiency.
Klaus Rajewsky, Max-Delbruck-Ctr. for Molecular Medicine
Homozygous mutant mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Homozygotes have a deficiency in the B-1 subset of B-lymphocytes along with a concomitant reduction in serum IgM. Their ability to respond to T-cell-dependent antigens is severely impaired, and they fail to form splenic germinal centers.
In addition to disrupting the targeted gene, the targeting construct also introduced acre cassette into exon 2 of the targeted gene, effectively placing cre expression under the control of the endogenous promoter. The Cd19 promoter specifically directs cre expression at the earliest stages and throughout B-lymphocyte development and differentiation. Although homozygous mutant mice are Cd19-deficient, heterozygous mice are phenotypically normal, and can be used for specific deletion of loxP-flanked (floxed) targets in B-lymphocytes.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing Cre recombinase, the rabbit beta-globin intron /poly A signal sequence, and an FRT-flanked neomycin resistance gene, was used to disrupt exon 2 of the Cd19 gene and to express cre under the regulation of the endogenous promoter. Herpes simplex virus thymidine kinase gene was placed 3' of the Cd19 sequence to allow for the selection against random integration. The construct was transfected into 129P2/OlaHsd-derived E14-1 embryonic stem cells. Correctly targeted ES cells were injected into 129/Sv blastocysts. The resulting chimeric animals were backcrossed to BALB/c IgHb congenics for a number of generations and then backcrossed to C57BL/6 for 10 generations.
|Expressed Gene||cre, cre recombinase, bacteriophage P1|
|Site of Expression||B cells|
|Allele Name||targeted mutation 1, University of Cologne|
|Allele Type||Targeted (Recombinase-expressing)|
|Allele Synonym(s)||B-Cre; CD19-; CD19-Cre; CD19-KO; Cd19Cre; Cd19cre|
|Gene Symbol and Name||Cd19, CD19 antigen|
|Gene Synonym(s)||AW495831; B4; CVID3; expressed sequence AW495831|
|Expressed Gene||cre, cre recombinase, bacteriophage P1|
|Site of Expression||B cells|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||In frame insertion of a cre recombinase gene into the first coding exon followed by an frt-flanked neomycin cassette. A frameshift mutation was also introduced into exon 5. This allele expresses cre recombinase specifically in B lineage cells throughout development.|
|Mutations Made By|| |
Robert Rickert, The Burnham Institute
When maintaining a live colony, these mice may be bred as heterozygotes or homozygotes. The donating investigator reports that homozygotes older than 3 months of age do not breed well. Homozygous breeding pairs maintained at The Jackson Laboratory have shown no such problems.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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