Overview

These transgenic mice express Cyan Fluorescent Protein (CFP) under the control of the mouse thymus cell antigen 1, theta, Thy1, promoter. CFP is specifically localized to the mitochondria by a human cytochrome c oxidase, subunit 8A (ubiquitous), targeting signal fused to the N-terminus. This mutant mouse strain may be useful in studies of mitochondrial transport.
This strain is discontinued. Search JAX^® Mice Database under "Strain Name/Common Name" using the search term "Thy1-CFP/COX8A" for other transgenic strains available similar to this strain.

Donating Investigator

Robert Burgess, The Jackson Laboratory

Genetic overview

Genetic Background	Generation

Tg(Thy1-CFP/COX8A)C1Lich

Allele Type
Transgenic (Reporter)

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Research Applications

Research Tools
Neurobiology Research
Cell Biology Research

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Details

Detailed Description

These transgenic mice express Cyan Fluorescent Protein (CFP) under the control of the mouse thymus cell antigen 1, theta, Thy1, promoter. CFP is specifically localized to the mitochondria by a human cytochrome c oxidase, subunit 8A (ubiquitous), targeting signal fused to the N-terminus. Fluorescence is detected in many neuronal populations including retinal cells and in all motor axons. Coronal brain sections reveal a fluorescence pattern showing somatosensory cortex barrel morphology. Neuronal, mitochondrial and neuromuscular junction morphology appears normal in transgenic mice. Axonal mitochondrial density is similar to wildtype. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of mitochondrial transport.

Development

A transgenic construct containing the entire open reading frame of the Cyan Fluorescent Protein (CFP) gene fused in-frame with the mitochondrial targeting sequence of the human cytochrome c oxidase subunit 8A (ubiquitous) gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized B6CBA mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder line C1, corresponding to the published line C, was subsequently established. The mice were backcrossed for an unknown number of generations to C57BL/6 and then for 1 generation to C57BL/6J after arriving at The Jackson Laboratory.

Expression Data

Expressed Gene	COX8A, cytochrome c oxidase subunit 8A, human
Expressed Gene	CFP, Cyan Fluorescent Protein, jellyfish
Site of Expression	mitochondria; many neuronal populations including retinal cells and all motor axons; coronal brain sections show pattern of somatosensory cortex barrel morphology

Control Suggestions

Noncarrier

Additional Information

Considerations for Choosing Controls

Selected References

Misgeld T; Kerschensteiner M; Bareyre FM; Burgess RW; Lichtman JW. 2007. Imaging axonal transport of mitochondria in vivo. Nat Methods 4(7):559-561PubMed: 17558414 MGI: J:122259

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Genetics

Tg(Thy1-CFP/COX8A)C1Lich

Allele Symbol: Tg(Thy1-CFP/COX8A)C1Lich

Allele Name	transgene insertion C1, Jeff W Lichtman
Allele Type	Transgenic (Reporter)
Allele Synonym(s)	Thy1-CFP-MitoC
Gene Symbol and Name	Tg(Thy1-CFP/COX8A)C1Lich, transgene insertion C1, Jeff W Lichtman
Gene Synonym(s)
Promoter	Thy1, thymus cell antigen 1, theta, mouse, laboratory
Expressed Gene	COX8A, cytochrome c oxidase subunit 8A, human
Expressed Gene	CFP, Cyan Fluorescent Protein, jellyfish
Site of Expression	mitochondria; many neuronal populations including retinal cells and all motor axons; coronal brain sections show pattern of somatosensory cortex barrel morphology
Strain of Origin	Not Applicable
Chromosome	UN
General Note	Founder line C1 corresponds to the published line C.
Molecular Note	A transgenic construct containing the entire open reading frame of the Cyan Fluorescent Protein (CFP) gene fused in-frame with the mitochondrial targeting sequence of the human cytochrome c oxidase subunit 8A (ubiquitous) gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized donor mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder line C1, corresponding to the published line C, was subsequently established.
Mutations Made By	Robert Burgess, The Jackson Laboratory

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Neurobiology Research
- Cell Biology Research
- Fluorescent Proteins
Neurobiology Research
- Fluorescent protein expression in neural tissue
Cell Biology Research
- Vesicular Trafficking

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tg(Thy1-CFP/COX8A)C1Lich/0
Not Specified

normal phenotype

no abnormal phenotype detected
- Normal - mice are viable and show no neurological abnormalities

References

Misgeld T; Kerschensteiner M; Bareyre FM; Burgess RW; Lichtman JW. 2007. Imaging axonal transport of mitochondria in vivo. Nat Methods 4(7):559-561PubMed: 17558414 MGI: J:122259

Additional - Tg(Thy1-CFP/COX8A)C1Lich related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Tg(Thy1-CFP/COX8A)C1Lich
- Probe:Fluorescent Proteins (Generic GFP)
- Standard PCR:Fluorescent Proteins (Generic GFP)
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice are bred as hemizygotes.
- Additional Breeding and Husbandry Support
Mating System
- Inbred x Hemizygote

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(Thy1-CFP/COX8A)C1Lich

Allele Symbol: Tg(Thy1-CFP/COX8A)C1Lich

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Tg(Thy1-CFP/COX8A)C1Lich/0Not Specified

normal phenotype

References

Additional - Tg(Thy1-CFP/COX8A)C1Lich related

Genotyping Protocols

Breeding Considerations

Mating System

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Genotype: Tg(Thy1-CFP/COX8A)C1Lich/0
Not Specified