These BDNF mice express a transgene containing the rat brain-derived neurotrophic factor driven by the Camk2a promoter affecting maturation and plasticity of the visual cortex.
Dr. Susumu Tonegawa, Massachusetts Institute of Technology
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Hemizygous mice are viable and fertile, although the donating investigator reports that hemizygous mice have breeding problems likely resulting from a social anxiety-related phenotype. These "BDNF (line A9)" mice express the rat brain-derived neurotrophic factor (BDNF) primarily in forebrain regions, including the neocortex and hippocampus. Weak transgenic BDNF expression is detected also in cerebellum. Within the primary visual cortex, transgene expression is highest in the superficial layers. Hemizygous mice exhibit accelerated maturation of GABAergic innervation and inhibition, earlier termination of the critical period for ocular dominance plasticity, and accelerated development of visual acuity. These transgenic mice may be useful for studies involving BDNF overexpression and synaptic maturation and plasticity in the visual cortex.
A rat Bdnf cDNA sequence (modified with a Kozac sequence inserted in front of the translation initiation codon) and a SV40 intron and polyadenylation sequence was placed downstream of the 8.5 kb promoter region from mouse Camk2a. This transgene was injected into the pronuclei of C57BL/6 embryos. The donating investigator reported that the resulting chimeric founders (line A9) were subsequently bred to C57BL/6 mice (see SNP note below) and maintained on that genetic background for many generations prior to arrival at The Jackson Laboratory.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Expressed Gene | Bdnf, brain-derived neurotrophic factor, rat |
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Site of Expression |
Allele Name | transgene insertion A9, Susumu Tonegawa |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Camk2a-Bdnf)A9Stl, transgene insertion A9, Susumu Tonegawa |
Gene Synonym(s) | |
Promoter | Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory |
Expressed Gene | Bdnf, brain-derived neurotrophic factor, rat |
Strain of Origin | (C57BL/6 x DBA/2)F2 |
Chromosome | UN |
Molecular Note | The transgene construct has the rat Bdnf cDNA sequence, with a Kozak sequence inserted in front of the translation initiation site, controlled by the mouse Camk2a promoter. The SV40 intron/polyA sequence was inserted the 3' to the Bdnf cDNA. ISH demonstrated that Bdnf overexpression is mainly restricted to the forebrain regions, including all of the neocortex, parts of the hippocampus, amygdala and striatum. |
Mutations Made By | Dr. Susumu Tonegawa, Massachusetts Institute of Technology |
When maintaining a live colony, hemizygotes are bred to wildtype siblings or to C57BL/6J inbred mice. The donating investigator reports that hemizygous mice have a breeding problem likely resulting from social anxiety phenotype.
When using the BDNF mouse strain in a publication, please cite the originating article(s) and include JAX stock #006579 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrierfor Tg(Camk2a-Bdnf)A9Stl |
Frozen Mouse Embryo | C57BL/6-Tg(Camk2a-Bdnf)A9Stl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Camk2a-Bdnf)A9Stl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Camk2a-Bdnf)A9Stl/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Camk2a-Bdnf)A9Stl/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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