These Syt1 knock-in mice exhibit altered Ca2+ protein affinity and sensitivity of neurotransmitter release. They are useful in studies of synaptic neurotransmission.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted | Syt1 | synaptotagmin I |
Mice homozygous for this targeted point mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. The mutant gene is fully expressed. A two-fold decrease in the overall Ca2+ affinity of the protein and a corresponding decrease in the Ca2+ sensitivity of neurotransmitter release is observed. Nuclear Magnetic Resonance (NMR) spectroscopy shows no structural or conformational changes in the mutant protein. This mutant mouse strain represents a model that may be useful in studies of synaptic neurotransmitter release.
A targeting vector was used to create an R233Q substitution in the exon encoding residues 214-269 and insert a floxed neomycin resistance gene into the flanking intron. The construct was electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6. The strain has been minimally backcrossed to C57BL/6 by the donating laboratory.
Allele Name | targeted mutation 3, Thomas C Sudhof |
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Allele Type | Targeted |
Allele Synonym(s) | R233Q; Syt1R233Q+ |
Gene Symbol and Name | Syt1, synaptotagmin I |
Gene Synonym(s) | |
Promoter | Syt1, synaptotagmin I, mouse, laboratory |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 10 |
Molecular Note | Insertion of a neomycin resistance cassette into intron 1 and an arginine to glutamine point mutation at position 233 of exon 1 (R233Q) was introduced via homologous recombination. Western blot of brain homogenates from homozygous mutant animals verified the presence of protein expression and enzyme assays demonstrated similar phospholipid- and syntaxin-binding ability compared to wild-type. However, calcium affinity was decreased by 2-fold. |
Mutations Made By | Dr. Thomas Sudhof, Stanford University School of Medicine |
When maintained as a live colony, homozygous breeders are crossed.
When using the R233Q KI mouse strain in a publication, please cite the originating article(s) and include JAX stock #006385 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Syt1<tm3Sud> |
Frozen Mouse Embryo | B6;129P2-Syt1<tm3Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Syt1<tm3Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Syt1<tm3Sud>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Syt1<tm3Sud>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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