B6.FVB-Tg(Cdh5-cre)7Mlia/J

Stock number: 006137
Product name: VE-Cadherin-Cre (VE-CRE)
Research areas: Cardiovascular Research, Developmental Biology Research, Hematological Research, Research Tools

Description

Cre expression in these transgenic mice can be seen in the embryo and in adult endothelium of developing and quiescent vessels, as well as in a subset of hematopoietic cells. When bred with mice carrying a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. These mice may be useful in studies of the cardiovascular system.

Detailed description

Hemizygotes and homozygotes are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. In the differentiated endothelium transgene expression is observed as early as E7.5 and progresses to almost full penetrance by E14.5. In adult mice, uniform cre expression is observed in the endothelium of developing and quiescent vessels of all organs examined, as well as within a subset of hematopoietic cells. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. These mice may be useful in studies of the cardiovascular system, including angiogenesis, and endothelial and hematopoietic cell lineages.

Cre activity is seen in 20%-40% of the fetal liver population and 52% of the adult bone marrow. These mice exhibit less efficient cre-mediated recombination than B6.Cg-Tg(Cdh5-cre)1Spe/J mice (Stock No. 033055), in which cre activity is seen in 85% of the fetal liver population and 96% of adult bone marrow.

View cre expression characterization.

Development

A recombinant vector was designed to place Cre recombinase cDNA downstream from a 2.5-kb fragment of the VE-Cadherin (Cdh5) mouse promoter. This construct was microinjected into FVB/N fertilized oocytes. A selected founder line ("VEN7-CRE") was established and then backcrossed to C57BL/6 for 12 generations prior to sending to The Jackson Laboratory Repository (in 2007). Upon arrival, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. After this, the colony was maintained for several generations by breeding transgenic mice together.

A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 27 markers, on Chromosome 4, was segregating with FVB. Also, 2 of the 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6N;C57BL/6J genetic background.

Allele

Symbol: Tg(Cdh5-cre)7Mlia
Name: transgene insertion 7, M Luisa Iruela-Arispe
MGI accession ID: MGI:3620560
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Cdh5-cre)7Mlia
Marker name: transgene insertion 7, M Luisa Iruela-Arispe
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Embryonic and adult expression in endothelium of developing and quiescent vessels of all organs examined, as well as within a subset of hematopoietic cells.
Molecular note: The cadherin 5 promoter was used to drive expression of cre recombinase in the adult epithelium. Three lines were created.