Cre expression in these transgenic mice can be seen in the embryo and in adult endothelium of developing and quiescent vessels, as well as in a subset of hematopoietic cells. When bred with mice carrying a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. These mice may be useful in studies of the cardiovascular system.
M. Luisa Iruela-Arispe, University of California, Los Angeles
Genetic Background | Generation |
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N12+N2F10
|
Allele Type |
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Transgenic (Recombinase-expressing) |
Hemizygotes and homozygotes are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. In the differentiated endothelium transgene expression is observed as early as E7.5 and progresses to almost full penetrance by E14.5. In adult mice, uniform cre expression is observed in the endothelium of developing and quiescent vessels of all organs examined, as well as within a subset of hematopoietic cells. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. These mice may be useful in studies of the cardiovascular system, including angiogenesis, and endothelial and hematopoietic cell lineages.
Cre activity is seen in 20%-40% of the fetal liver population and 52% of the adult bone marrow. These mice exhibit less efficient cre-mediated recombination than B6.Cg-Tg(Cdh5-cre)1Spe/J mice (Stock No. 017968), in which cre activity is seen in 85% of the fetal liver population and 96% of adult bone marrow.
View cre expression characterization.
A recombinant vector was designed to place Cre recombinase cDNA downstream from a 2.5-kb fragment of the VE-Cadherin (Cdh5) mouse promoter. This construct was microinjected into FVB/N fertilized oocytes. A selected founder line ("VEN7-CRE") was established and then backcrossed to C57BL/6 for 12 generations prior to sending to The Jackson Laboratory Repository (in 2007). Upon arrival, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. After this, the colony was maintained for several generations by breeding transgenic mice together.
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 27 markers, on Chromosome 4, was segregating with 129. Also, 2 of the 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6N;C57BL/6J genetic background.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Embryonic and adult expression in endothelium of developing and quiescent vessels of all organs examined, as well as within a subset of hematopoietic cells. |
Allele Name | transgene insertion 7, M Luisa Iruela-Arispe |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Tg(Cdh5-cre)1Mlia; VE-Cadherin-Cre; VEC-Cre; VEN7-CRE |
Gene Symbol and Name | Tg(Cdh5-cre)7Mlia, transgene insertion 7, M Luisa Iruela-Arispe |
Gene Synonym(s) | |
Promoter | Cdh5, cadherin 5, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Embryonic and adult expression in endothelium of developing and quiescent vessels of all organs examined, as well as within a subset of hematopoietic cells. |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The cadherin 5 promoter was used to drive expression of cre recombinase in the adult epithelium. Three lines were created. |
Mutations Made By | M. Luisa Iruela-Arispe, University of California, Los Angeles |
When maintaining a live colony, hemizygotes can be bred to wildtype siblings, C57BL/6J, or bred together to generate homozygous colonies.
When using the VE-Cadherin-Cre (VE-CRE) mouse strain in a publication, please cite the originating article(s) and include JAX stock #006137 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(Cdh5-cre)7Mlia |
Frozen Mouse Embryo | B6.FVB-Tg(Cdh5-cre)7Mlia/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.FVB-Tg(Cdh5-cre)7Mlia/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.FVB-Tg(Cdh5-cre)7Mlia/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.FVB-Tg(Cdh5-cre)7Mlia/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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