These transgenic mice harbor the human growth hormone gene driven by Β-globin regulatory elements, and exhibit greater bone density and increased cortical bone thickness than controls.
Donna King, N East Ohio Univ Coll of Med (NEOUCOM)
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Mice hemizygous for this transgene are viable. While males are fertile, transgenic females are unreliable breeders and mothers. Homozygotes are sterile. In both fetal and adult tissues, erythroid-specific expression of the transgene is observed. Transgenic mice have 120-150% greater mature body mass and increased bone mass. The donating investigator also reports transgenic mice have increased dermatological stiffness and toughness. Transgenic mice have a characteristic coat with few guard hairs compared to wildtype littermates. These mice may be useful in studies of bone formation and development, dermatology, and acromegaly.
The human growth hormone gene was modified by replacing the endogenous human promoter with a sheep beta-C globin (HBB) promoter sequence proximal to the 5' human beta-globin locus control region (HS-2). The resulting transgene (termed HS-2+betachGH) was injected into the pronucleus of (C57BL/6J x SJL)F1 embryos. Embryos were transferred into pseudopregnant foster mothers. Transgenic offspring (founder line TG420) were bred to C57BL/6J females for approximately 20 generations prior to arrival at The Jackson Laboratory.
Expressed Gene | GH1, growth hormone 1, human |
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Site of Expression |
Allele Name | transgene insertion 420, Donna King |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | HS-2 + beta-c hGH; Tg420 |
Gene Symbol and Name | Tg(HBB-GH1)420King, transgene insertion 420, Donna King |
Gene Synonym(s) | |
Promoter | HBB, Hemoglobin subunit beta, sheep |
Expressed Gene | GH1, growth hormone 1, human |
Strain of Origin | (C57BL/6J x SJL/J)F2 |
Chromosome | UN |
Molecular Note | The human genomic growth hormone gene, from its transcription initiation site through the 3' untranslated region, was placed under control of the sheep hemoglobin betac promoter joined at its 5' end to a 1.9-kb fragment containing the 5' HS-2 element of the human hemoglobin beta locus control region. Radiolabeled human growth hormone antisense RNA detects transcripts in liver and heart (in the latter presumably from pooled blood) of whole-embryo sections and in bone marrow of sectioned adult tibiae. |
Mutations Made By | Donna King, N East Ohio Univ Coll of Med (NEOUCOM) |
When maintaining a live colony, transgenic males are bred to C57BL/6J or wildtype siblings. The donating investigator reports that transgenic females are unreliable breeders and can exhibit poor fostering of pups. If breeding transgenic females, breed as young as possible and be prepared to foster pups.
When using the TG420 mouse strain in a publication, please cite the originating article(s) and include JAX stock #006086 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(HBB-GH1)420King |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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