Overview

Also Known As:NOD.DQ6, H2-Ab0^null,Rag1^null

These NOD mice carry human CD4 and HLA-DQ6 transgenes resulting in a lack of T and B cells.

Donating Investigator

John Elliott, University of Alberta

Genetic overview

Genetic Background	Generation
001976 NOD/ShiLtJ

Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell

Allele Type
Transgenic (Inserted expressed sequence, Humanized sequence)

Rag1^tm1Mom

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Rag1	recombination activating gene 1

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	H2-Ab1	histocompatibility 2, class II antigen A, beta 1

Research Applications

Cancer Research
Hematological Research
Immunology, Inflammation and Autoimmunity Research
Research Tools
Internal/Organ Research
Diabetes and Obesity Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

Rag1 and H2-Ab1 deficient NOD mice carrying transgenes encoding humanized CD4 and HLA-DQ6 lack T and B cells and are free of autoimmune diabetes and cardiomyopathy, and do not develop thyroiditis.

This model is useful in adoptive transfer experiments and in studying MHC class II-based resistance and susceptibility in autoimmunity.

Development

Congenic, H2-Ab1 deficient, NOD mice carrying transgenes encoding CD4 and a human genomic fragment containing the entire HLA-DQ6 gene (DQA1*0102, DQB1*0602, Stock No. 006023) were crossed to congenic Rag1 deficient NOD mice (Stock No. 003729), and were bred to homozygosity for the H2-Ab1^tm1Gru and Rag1^tm1Mom targeted mutations. The T1DR received this strain in 2006.

Expression Data

Expressed Gene	HLA-DQA1, major histocompatibility complex, class II, DQ alpha 1, human
Expressed Gene	CD4, CD4 molecule, human
Expressed Gene	HLA-DQB1, major histocompatibility complex, class II, DQ beta 1, human
Site of Expression
Site of Expression	expression is seen in bone marrow derived cell lines.
Site of Expression

Control Suggestions

001976 NOD/ShiLtJ

Additional Information

Considerations for Choosing Controls

Selected References

Hayward SL; Suzuki K; Elliott JF. 2007. A radioligand binding assay to measure anti-thyroperoxidase autoantibodies in mice. J Immunol Methods 323(2):114-22PubMed: 17482640 MGI: J:124227

View All References

Genetics

Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell

Allele Symbol: Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell

Allele Name	transgene insertion N8, John F Elliott
Allele Type	Transgenic (Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	DQ6 transgene; HLA-DQ6 transgene; Tg(CD4,HLA-DQA10102,HLADQB10602)1Ell
Gene Symbol and Name	Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell, transgene insertion N8, John F Elliott
Gene Synonym(s)
Promoter	HLA-DQA1, major histocompatibility complex, class II, DQ alpha 1, human
Promoter	HLA-DQB1, major histocompatibility complex, class II, DQ beta 1, human
Expressed Gene	HLA-DQA1, major histocompatibility complex, class II, DQ alpha 1, human
Expressed Gene	CD4, CD4 molecule, human
Expressed Gene	HLA-DQB1, major histocompatibility complex, class II, DQ beta 1, human
Strain of Origin	NOD/ShiLtJ
Chromosome	UN
Molecular Note	The human CD4 gene lacking one exon but including its promoter was fused downstream of a mouse CD4 enhancer element then mixed with a large DNA fragment containing both HLA-DQA0102 and HLA-DQB0602 and injected into NOD/LtJ one celled embryos. A single founder line is maintained.

Rag1^tm1Mom

Allele Symbol: Rag1^tm1Mom

Allele Name	targeted mutation 1, Peter Mombaerts
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Rag-; Rag^1tm1Mom; RAG-1-; Rag1^-; RAG1^null; Rag-1KO
Gene Symbol and Name	Rag1, recombination activating gene 1
Gene Synonym(s)
Site of Expression	expression is seen in bone marrow derived cell lines.
Strain of Origin	129S7/SvEvBrd-Hprt⁺
Chromosome	2
Molecular Note	A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice.
Mutations Made By	Peter Mombaerts, Max Planck Research Unit for Neurogenetics

H2-Ab1^tm1Gru

Allele Symbol: H2-Ab1^tm1Gru

Allele Name	targeted mutation 1, Michael J Grusby
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	[KO]Ab^b; A^bbeta^-; Ab1^tm1Gru; Abb-; Abb^tm1; Abeta-; Abeta^b-; C2-; H2-Ab1^-; H2-Ab1^tm1Glm; I-A^b-; IAb^-; IAbeta-; I-Abeta^b-; MHC class II-; MHC-II-deficient; mII^-
Gene Symbol and Name	H2-Ab1, histocompatibility 2, class II antigen A, beta 1
Gene Synonym(s)
Strain of Origin	129S2/SvPas
Chromosome	17
Molecular Note	A neomycin selection cassette was inserted into exon 2. In addition, the ES cell line used was derived from the 129S2/SvPas strain, which carries a deletion in the promoter region of H2-Ea. Consequently, these MHC class II molecule-deficient mice lacked cell surface expression of both class II-A and class II-E MHC proteins.
Mutations Made By	Dr. Michael Grusby, Harvard Medical School

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Rag1^tm1Mom related

Cancer Research
- Toxicology
  - B and T cell deficiency, xenograft/transplant host
Hematological Research
- Immunological Defects
  - B and T cell deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B and T cell deficiency
- T Cell Receptor Signaling Defects
  - B and T cell deficiency
- Inflammation
  - B and T cell deficiency
Research Tools
- Toxicology Research
  - xenograft/transplant host
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency

Genotype: H2-Ab1^tm1Gru related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
- Immunodeficiency

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - MHC class II defects
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - MHC class II deficient
- HLA transgenics
Diabetes and Obesity Research
- Islet Transplantation Studies
- Type 1 Diabetes (IDDM) Analysis Strains
  - NOD Transgenics
  - NOD Congenics with Mutations Affecting Immunocompetence

Mammalian Phenotype Terms by Genotype

References

Hayward SL; Suzuki K; Elliott JF. 2007. A radioligand binding assay to measure anti-thyroperoxidase autoantibodies in mice. J Immunol Methods 323(2):114-22PubMed: 17482640 MGI: J:124227

Additional - H2-Ab1^tm1Gru related

Additional - Rag1^tm1Mom related

Additional - Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell
- Separated PCR:H2-Ab1alternate2
- Standard PCR:Rag1Alternate1
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, mice homozygous for both targeted mutations and hemizygous for the transgene may be bred together. These mice are immunodeficient and need to be maintained in a high barrier environment with sterile food and water.

Rag1-deficient, H2-Ab1-deficient mice are immunodeficient. As such, and similar to other immunodeficient strains, maintenance in high health status (specific pathogen-free) vivaria promotes overall colony health. If these animals are maintained in low health barrier rooms, the use of medicated water (e.g., sulfatrim/trimethoprim-sulfa or enrofloxacin/Baytril) is suggested to increase overall colony health.
- Additional Breeding and Husbandry Support
Appearance
- pink-eyed, albino
Citation
When using the NOD.DQ6, H2-Ab0^null,Rag1^null mouse strain in a publication, please cite the originating article(s) and include JAX stock #006024 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Not-For-Profit & Academic Pricing

Service/Product	Description	Price
	Homozygous for Rag1<tm1Mom>, Homozygous for H2-Ab1<tm1Gru>, Hemizygous (Tg/0) or Homozygous or Non carrier for Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

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Also Known As:NOD.DQ6, H2-Ab0null,Rag1null

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell

Allele Symbol: Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell

Rag1tm1Mom

Allele Symbol: Rag1tm1Mom

H2-Ab1tm1Gru

Allele Symbol: H2-Ab1tm1Gru

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Rag1tm1Mom related

Genotype: H2-Ab1tm1Gru related

Mammalian Phenotype Terms by Genotype

References

Additional - H2-Ab1tm1Gru related

Additional - Rag1tm1Mom related

Additional - Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell related

Genotyping Protocols

Breeding Considerations

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Not-For-Profit & Academic Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As:NOD.DQ6, H2-Ab0^null,Rag1^null

Rag1^tm1Mom

Allele Symbol: Rag1^tm1Mom

H2-Ab1^tm1Gru

Allele Symbol: H2-Ab1^tm1Gru

Genotype: Rag1^tm1Mom related

Genotype: H2-Ab1^tm1Gru related

Additional - H2-Ab1^tm1Gru related

Additional - Rag1^tm1Mom related