C3Bir.129P2(B6)-Il10^C3Bir/LtJ

Stock number: 005973
Product name: IL10T
Research areas: Immunology, Inflammation and Autoimmunity Research

Detailed description

This is a control strain for C3Bir.129P2(B6)-Il10^tm1Cgn/LtJ (Stock No. 003968); it does not exhibit the Il10-deficient phenotype.

Development

This is a control strain for the Il10 knock-out strain, C3Bir.129P2(B6)-Il10^tm1Cgn/LtJ (originally Stock No. 4326 that is now extinct); superseded by Stock No. 003968), and was developed in parallel with the mutant strain until backcrossing was complete.

The Il10^tm1Cgn knock-out mutation was created by in the Laboratory of Dr. Werner Muller at the University of Cologne. Briefly, a targeting vector was designed to replace codons 5-55 of exon 1 of the targeted gene with a 24 bp linker (providing a termination codon) and a neo expression cassette, as well as introduce a termination codon into exon 3. The construct was electroporated into 129P2/OlaHsd-derived E14-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females to establish the mutant colony on a mixed B6;129P2 genetic background. It was then imported to the Induced Mutant Resource at The Jackson Laboratory in 1995. Subsequently, mutant mice were backcrossed to the C3H/HeJBirLt host strain for at least 10 generations. At the tenth backcross generation, heterozygotes were intercrossed to produce founders for the homozygous C3Bir.129P2(B6)-Il10^tm1Cgn/Lt mutant line as well as founders for this wildtype control line (Stock No. 005973). This strain was imported into The Jackson Laboratory Repository at N15F15 from the research laboratory of Dr. Ed Leiter in 2006.

Allele

Symbol: Tlr4^Lps-d
Name: defective lipopolysaccharide response
MGI accession ID: MGI:1857718
Generation method: Spontaneous
Marker symbol: Tlr4
Marker name: toll-like receptor 4
Chromosome: 4
Strain of origin: C3H/HeJ
Molecular note: This allele corresponds to a mutation in the third exon of the gene. A C-to-A substitution at coding tide position 2135 (c.2135C>A) results in an amino acid substitution that replaces proline with histidine at position 712 (p.P712H).
General note: C3H/HeJ mice carry this allele. Various combinations of Lps-associated traits have been followed in crosses between C3H/HeJ and other C3H substrains, and the traits have in all cases segregated together (J:30692, J:5557, J:5593, J:5938). Some of the traits show dominance of the Tlr4^lps-n allele; others, including Tlr4^Lps-d, show codominance.

Genbank ID for this allele: AF095353

Allele

MGI accession ID: MGI:96537
Marker symbol: Il10
Marker name: interleukin 10
Chromosome: 1