This is a control strain for C3Bir.129P2(B6)-Il10tm1Cgn/LtJ (Stock No. 003968); it does not exhibit the Il10-deficient phenotype.
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Tlr4 | toll-like receptor 4 |
Marker Symbol | Marker Name | |
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Il10 | interleukin 10 |
This strain is homozygous for Pde6brd1 and Tlr4Lps-d.
This is a control strain for C3Bir.129P2(B6)-Il10tm1Cgn/LtJ (Stock No. 003968); it does not exhibit the Il10-deficient phenotype.
This is a control strain for the Il10 knock-out strain, C3Bir.129P2(B6)-Il10tm1Cgn/LtJ (originally Stock No. 4326 that is now extinct); superseded by Stock No. 003968), and was developed in parallel with the mutant strain until backcrossing was complete.
The Il10tm1Cgn knock-out mutation was created by in the Laboratory of Dr. Werner Muller at the University of Cologne. Briefly, a targeting vector was designed to replace codons 5-55 of exon 1 of the targeted gene with a 24 bp linker (providing a termination codon) and a neo expression cassette, as well as introduce a termination codon into exon 3. The construct was electroporated into 129P2/OlaHsd-derived E14-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females to establish the mutant colony on a mixed B6;129P2 genetic background. It was then imported to the Induced Mutant Resource at The Jackson Laboratory in 1995. Subsequently, mutant mice were backcrossed to the C3H/HeJBirLt host strain for at least 10 generations. At the tenth backcross generation, heterozygotes were intercrossed to produce founders for the homozygous C3Bir.129P2(B6)-Il10tm1Cgn/Lt mutant line as well as founders for this wildtype control line (Stock No. 005973). This strain was imported into The Jackson Laboratory Repository at N15F15 from the research laboratory of Dr. Ed Leiter in 2006.
Allele Name | defective lipopolysaccharide response |
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Allele Type | Spontaneous |
Allele Synonym(s) | lpsd; mutant TLR4; TlrLps-d; Tlr4-; Tlr4d; TLR4lps-def; TLR4d; TLR4-M; TLR4-Mu |
Gene Symbol and Name | Tlr4, toll-like receptor 4 |
Gene Synonym(s) | |
Strain of Origin | C3H/HeJ |
Chromosome | 4 |
General Note | C3H/HeJ mice carry this allele. Various combinations of Lps-associated traits have been followed in crosses between C3H/HeJ and other C3H substrains, and the traits have in all cases segregated together (J:30692, J:5557, J:5593, J:5938). Some of the traits show dominance of the Tlr4lps-n allele; others, including Tlr4Lps-d, show codominance. Genbank ID for this allele: AF095353 |
Molecular Note | This allele corresponds to a mutation in the third exon of the gene. A C-to-A substitution at coding tide position 2135 (c.2135C>A) results in an amino acid substitution that replaces proline with histidine at position 712 (p.P712H). |
Marker Synonym(s) | |
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Chromosome(s) | 1 |
When maintaining a live colony, homozygous mice may be bred together.
When using the IL10T mouse strain in a publication, please cite the originating article(s) and include JAX stock #005973 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous, 1 pair minimum |
Frozen Mouse Embryo | C3Bir.129P2(B6)-Il10<C3Bir>/LtJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C3Bir.129P2(B6)-Il10<C3Bir>/LtJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C3Bir.129P2(B6)-Il10<C3Bir>/LtJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C3Bir.129P2(B6)-Il10<C3Bir>/LtJ Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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