C57BL/6J-Tg(Mt1-Tnfsf4)1Pgn/Pgn

Stock number: 005967
Product name: Tnfsf4 transgenic1
Research areas: Cardiovascular Research

Description

These Tnfsf4 transgenic mice express much higher levels of Tnfsf4 mRNA than do non-transgenic littermate controls.

Detailed description

Transgenic mice are susceptible to high fat diet-induced atherosclerosis as compared to C57BL/6 controls. Wang (Wang X, et al., Nat Genet, 2005) characterizes Tg(Mt1-Tnfsf4)2Pgn, however, Tg(Mt1-Tnfsf4)1Pgn has a similar phenotype (personal communication).

Development

Mice were developed on the C57BL/6J background using a transgenic construct containing a metallothionien promoter, a beta globin IVS intron and a 0.87 Kb fragment of C57BL/6 mouse Tnfsf4 cDNA.

Allele

Symbol: Tg(Mt1-Tnfsf4)1Pgn
Name: transgene insertion 1, Beverly Paigen
MGI accession ID: MGI:3626317
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Mt1-Tnfsf4)1Pgn
Marker name: transgene insertion 1, Beverly Paigen
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: Tnfsf4,tumor necrosis factor (ligand) superfamily, member 4,mouse, laboratory
Molecular note: The Tnfsf4 cDNA, amplified from mRNA isolated from spleens of C57BL/6J mice, was placed under control of the ubiquitously expressed metallothionein promoter followed by the hemoglobin beta IVS intron. The 3' end of the cDNA, without its polyadenylation signal, was joined to a fragment of the human growth hormone gene, which distinguishes transgene-derived from endogenous transcripts. Transgenic mice express 4- to 250-fold higher levels, depending on tissue type, of transgenic mRNA than do nontransgenic littermates in liver, aorta, heart and spleen.
General note: This line is representative of four transgenic lines. According to the authors, although the line 2 was the primary one described in the reference, all four share the same phenotype.