These Tnfsf4 transgenic mice express much higher levels of Tnfsf4 mRNA than do non-transgenic littermate controls.
Read More +Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Transgenic mice are susceptible to high fat diet-induced atherosclerosis as compared to C57BL/6 controls. Wang (Wang X, et al., Nat Genet, 2005) characterizes Tg(Mt1-Tnfsf4)2Pgn, however, Tg(Mt1-Tnfsf4)1Pgn has a similar phenotype (personal communication).
Mice were developed on the C57BL/6J background using a transgenic construct containing a metallothionien promoter, a beta globin IVS intron and a 0.87 Kb fragment of C57BL/6 mouse Tnfsf4 cDNA.
Expressed Gene | Tnfsf4, tumor necrosis factor (ligand) superfamily, member 4, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 1, Beverly Paigen |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Mt1-Tnfsf4)1Pgn, transgene insertion 1, Beverly Paigen |
Gene Synonym(s) | |
Promoter | Mt1, metallothionein 1, mouse, laboratory |
Expressed Gene | Tnfsf4, tumor necrosis factor (ligand) superfamily, member 4, mouse, laboratory |
Strain of Origin | C57BL/6J |
Chromosome | UN |
General Note | This line is representative of four transgenic lines. According to the authors, although the line 2 was the primary one described in the reference, all four share the same phenotype. |
Molecular Note | The Tnfsf4 cDNA, amplified from mRNA isolated from spleens of C57BL/6J mice, was placed under control of the ubiquitously expressed metallothionein promoter followed by the hemoglobin beta IVS intron. The 3' end of the cDNA, without its polyadenylation signal, was joined to a fragment of the human growth hormone gene, which distinguishes transgene-derived from endogenous transcripts. Transgenic mice express 4- to 250-fold higher levels, depending on tissue type, of transgenic mRNA than do nontransgenic littermates in liver, aorta, heart and spleen. |
When using the Tnfsf4 transgenic1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #005967 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Mt1-Tnfsf4)1Pgn |
Frozen Mouse Embryo | C57BL/6J-Tg(Mt1-Tnfsf4)1Pgn/Pgn Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(Mt1-Tnfsf4)1Pgn/Pgn Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(Mt1-Tnfsf4)1Pgn/Pgn Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C57BL/6J-Tg(Mt1-Tnfsf4)1Pgn/Pgn Frozen Embryos | $3373.50 |
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