JAX Mouse Strain Datasheet - 005807

B6.129P2-Gpr6^tm1Dgen/J

Stock No:: 005807

Cryo Recovery

Overview

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Genetic overview

Genetic Background	Generation

Gpr6^tm1Dgen

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout, Reporter)	Gpr6	G protein-coupled receptor 6

View Genetics

Research Applications

Neurobiology Research
Research Tools

View All Research Applications

Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Development

The construct insert is reported to be "Lac0-SA-IRES-lacZ-Neo555G/Kan." Upon arrival, these mice had been backcrossed four generations to C57BL/6. During the importation process at The Jackson Laboratory, at least one additional backcross to C57BL/6J mice was performed. The resulting mice are backcrossed a total of at least five generations.

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ expression mimics endogenous gene expression and is detected in the brain, eyes and pituitary gland.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Genetics

Gpr6^tm1Dgen

Allele Symbol: Gpr6^tm1Dgen

Allele Name	targeted mutation 1, Deltagen
Allele Type	Targeted (Null/Knockout, Reporter)
Allele Synonym(s)
Gene Symbol and Name	Gpr6, G protein-coupled receptor 6
Gene Synonym(s)	AI852874; Gm233; expressed sequence AI852874; gene model 233, (NCBI)
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ expression mimics endogenous gene expression and is detected in the brain, eyes and pituitary gland.
Strain of Origin	129P2/OlaHsd
Chromosome	10
Molecular Note	A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects a high level of gene transcript in the whole brain, cortex, subcortical region, pancreas, pituitary gland and adrenal gland, moderate levels in cerebellum, olfactory bulb, eye, heart, liver, thymus, lymph nodes, skin, salivary gland, large intestine and prostate gland and low levels in kidney, spleen, testis and seminal vesicle. Beta-galactosidase expression from this allele is detectable in brain, eyes and pituitary gland. Strong expression was detected in several regions of the brain, including the caudate putamen, cortex, thalamus and hippocampus. Moderate expression was detected in some cells in the inner nuclear layer of the retina. In the pituitary gland, moderate staining was observed in many cells of the pars intermedia.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Receptor Defects
  - G protein-coupled receptor
Research Tools
- lacZ Expression

Mammalian Phenotype Terms by Genotype

Genotype: Gpr6^tm1Dgen/Gpr6^tm1Dgen
B6.129P2-Gpr6^tm1Dgen

behavior/neurological phenotype

abnormal associative learning
- however, sensitivity to devaluation in instrumental conditioning remains normal and appetite motivated response to auditory cued conditioning is normal
- instrumental-conditioning is reduced compared to in wild-type mice that acquire the bar-pressing response to obtain food faster and reach higher asymptotic performance after more extensive training
- mice are more sensitive to devaluation than wild-type mice in an auditory cued conditioning experiment
- under a progressive ratio 5 schedule, mice respond at higher rates and earn more food reward than wild-type mice and in omission testing mice continue to press the level compared to wild-type mice that stop

nervous system phenotype

abnormal striatum morphology
- however, forskolin-induced camp production is normal
- plasma membrane extracts from the striatum contain 55% less cAMP that in wild-type mice

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Gpr6^tm1Dgen/Gpr6^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6

normal phenotype

no abnormal phenotype detected
- no significant differences in physical examinations, necropsy including body length, body weight, and organ weight measurements, histological examination of tissues and organs, bone marrow section evaluations, complete blood counts and differentials, clinical chemistry panels, densitometry or behavioral tests detected in the homozygous mutant mice when compared with age- and gender-matched wild-type control miceical chemistry panels, densitometry or behavioral tests detected in the homozygous mutant mice when compared with age- and gender-matched wild-type control mice

References

Additional - Gpr6^tm1Dgen related

Deltagen Inc. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data Submission :. MGI: J:101679
Lobo MK; Cui Y; Ostlund SB; Balleine BW; William Yang X. 2007. Genetic control of instrumental conditioning by striatopallidal neuron-specific S1P receptor Gpr6. Nat Neurosci 10(11):1395-1397. PubMed: 17934457 MGI: J:126659
Oeckl P; Ferger B. 2016. Increased susceptibility of G-protein coupled receptor 6 deficient mice to MPTP neurotoxicity. Neuroscience 337:218-223. PubMed: 27651149 MGI: J:238215
Oeckl P; Hengerer B; Ferger B. 2014. G-protein coupled receptor 6 deficiency alters striatal dopamine and cAMP concentrations and reduces dyskinesia in a mouse model of Parkinson's disease. Exp Neurol 257:1-9. PubMed: 24747358 MGI: J:216260

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Wild-type for Gpr6<tm1Dgen>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by academics, non-profits, companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Gpr6tm1Dgen

Allele Symbol: Gpr6tm1Dgen

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Gpr6tm1Dgen/Gpr6tm1DgenB6.129P2-Gpr6tm1Dgen

behavior/neurological phenotype

nervous system phenotype

Genotype: Gpr6tm1Dgen/Gpr6tm1Dgeninvolves: 129P2/OlaHsd * C57BL/6

normal phenotype

References

Additional - Gpr6tm1Dgen related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Gpr6^tm1Dgen

Allele Symbol: Gpr6^tm1Dgen

Genotype: Gpr6^tm1Dgen/Gpr6^tm1Dgen
B6.129P2-Gpr6^tm1Dgen

Genotype: Gpr6^tm1Dgen/Gpr6^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6

Additional - Gpr6^tm1Dgen related