B6.129P2-Gpr6^tm1Dgen/J

Stock number: 005807
Research areas: Neurobiology Research, Research Tools

Detailed description

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Development

The construct insert is reported to be "Lac0-SA-IRES-lacZ-Neo555G/Kan." Upon arrival, these mice had been backcrossed four generations to C57BL/6. During the importation process at The Jackson Laboratory, at least one additional backcross to C57BL/6J mice was performed. The resulting mice are backcrossed a total of at least five generations.

Allele

Symbol: Gpr6^tm1Dgen
Name: targeted mutation 1, Deltagen
MGI accession ID: MGI:3604582
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Gpr6
Marker name: G protein-coupled receptor 6
Chromosome: 10
Strain of origin: 129P2/OlaHsd
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ expression mimics endogenous gene expression and is detected in the brain, eyes and pituitary gland.
Molecular note: A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects a high level of gene transcript in the whole brain, cortex, subcortical region, pancreas, pituitary gland and adrenal gland, moderate levels in cerebellum, olfactory bulb, eye, heart, liver, thymus, lymph nodes, skin, salivary gland, large intestine and prostate gland and low levels in kidney, spleen, testis and seminal vesicle. Beta-galactosidase expression from this allele is detectable in brain, eyes and pituitary gland. Strong expression was detected in several regions of the brain, including the caudate putamen, cortex, thalamus and hippocampus. Moderate expression was detected in some cells in the inner nuclear layer of the retina. In the pituitary gland, moderate staining was observed in many cells of the pars intermedia.