This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Gpr6 | G protein-coupled receptor 6 |
This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.
The construct insert is reported to be "Lac0-SA-IRES-lacZ-Neo555G/Kan." Upon arrival, these mice had been backcrossed four generations to C57BL/6. During the importation process at The Jackson Laboratory, at least one additional backcross to C57BL/6J mice was performed. The resulting mice are backcrossed a total of at least five generations.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | lacZ expression mimics endogenous gene expression and is detected in the brain, eyes and pituitary gland. |
Allele Name | targeted mutation 1, Deltagen |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Gpr6, G protein-coupled receptor 6 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ expression mimics endogenous gene expression and is detected in the brain, eyes and pituitary gland. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 10 |
Molecular Note | A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects a high level of gene transcript in the whole brain, cortex, subcortical region, pancreas, pituitary gland and adrenal gland, moderate levels in cerebellum, olfactory bulb, eye, heart, liver, thymus, lymph nodes, skin, salivary gland, large intestine and prostate gland and low levels in kidney, spleen, testis and seminal vesicle. Beta-galactosidase expression from this allele is detectable in brain, eyes and pituitary gland. Strong expression was detected in several regions of the brain, including the caudate putamen, cortex, thalamus and hippocampus. Moderate expression was detected in some cells in the inner nuclear layer of the retina. In the pituitary gland, moderate staining was observed in many cells of the pars intermedia. |
When using the B6.129P2-Gpr6tm1Dgen/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005807 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Gpr6<tm1Dgen> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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