Overview

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Genetic overview

Genetic Background	Generation

Gpr6^tm1Dgen

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Gpr6	G protein-coupled receptor 6

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Research Applications

Research Tools
Neurobiology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Development

The construct insert is reported to be "Lac0-SA-IRES-lacZ-Neo555G/Kan." Upon arrival, these mice had been backcrossed four generations to C57BL/6. During the importation process at The Jackson Laboratory, at least one additional backcross to C57BL/6J mice was performed. The resulting mice are backcrossed a total of at least five generations.

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ expression mimics endogenous gene expression and is detected in the brain, eyes and pituitary gland.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Genetics

Gpr6^tm1Dgen

Allele Symbol: Gpr6^tm1Dgen

Allele Name	targeted mutation 1, Deltagen
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	targeted mutation 1, Deltagen; Gpr6^tm1Dgen
Gene Symbol and Name	Gpr6, G protein-coupled receptor 6
Gene Synonym(s)	Gm233; gene model 233, (NCBI); AI852874; expressed sequence AI852874
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ expression mimics endogenous gene expression and is detected in the brain, eyes and pituitary gland.
Strain of Origin	129P2/OlaHsd
Chromosome	10
Molecular Note	A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects a high level of gene transcript in the whole brain, cortex, subcortical region, pancreas, pituitary gland and adrenal gland, moderate levels in cerebellum, olfactory bulb, eye, heart, liver, thymus, lymph nodes, skin, salivary gland, large intestine and prostate gland and low levels in kidney, spleen, testis and seminal vesicle. Beta-galactosidase expression from this allele is detectable in brain, eyes and pituitary gland. Strong expression was detected in several regions of the brain, including the caudate putamen, cortex, thalamus and hippocampus. Moderate expression was detected in some cells in the inner nuclear layer of the retina. In the pituitary gland, moderate staining was observed in many cells of the pars intermedia.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- lacZ Expression
Neurobiology Research
- Receptor Defects
  - G protein-coupled receptor

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Gpr6^tm1Dgen/Gpr6^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6

normal phenotype

no abnormal phenotype detected
- Normal - ical chemistry panels, densitometry or behavioral tests detected in the homozygous mutant mice when compared with age- and gender-matched wild-type control mice
- Normal - no significant differences in physical examinations, necropsy including body length, body weight, and organ weight measurements, histological examination of tissues and organs, bone marrow section evaluations, complete blood counts and differentials, clinical chemistry panels, densitometry or behavioral tests detected in the homozygous mutant mice when compared with age- and gender-matched wild-type control mice

The following phenotype relates to a compound genotype created using this strain

Genotype: Gpr6^tm1Dgen/Gpr6^tm1Dgen
B6.129P2-Gpr6

behavior/neurological phenotype

abnormal associative learning
- Normal - however, sensitivity to devaluation in instrumental conditioning remains normal and appetite motivated response to auditory cued conditioning is normal
- instrumental-conditioning is reduced compared to in wild-type mice that acquire the bar-pressing response to obtain food faster and reach higher asymptotic performance after more extensive training
- mice are more sensitive to devaluation than wild-type mice in an auditory cued conditioning experiment
- under a progressive ratio 5 schedule, mice respond at higher rates and earn more food reward than wild-type mice and in omission testing mice continue to press the level compared to wild-type mice that stop

nervous system phenotype

abnormal striatum morphology
- Normal - however, forskolin-induced camp production is normal
- plasma membrane extracts from the striatum contain 55% less cAMP that in wild-type mice

References

Additional - Gpr6^tm1Dgen related

Technical Support

Contact Technical Support

Genotyping Protocols
- SEPARATED MELT: Gpr6^tm1Dgen Alternate1
- Genotyping resources and troubleshooting
Citation
When using the B6.129P2-Gpr6^tm1Dgen/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005807 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Wild-type for Gpr6<tm1Dgen>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

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Licensing Information

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JAX® Mice, Products & Services Conditions of Use

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Animal Care and Use for SERVICES

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Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Gpr6tm1Dgen

Allele Symbol: Gpr6tm1Dgen

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Gpr6tm1Dgen/Gpr6tm1Dgeninvolves: 129P2/OlaHsd * C57BL/6

normal phenotype

Genotype: Gpr6tm1Dgen/Gpr6tm1DgenB6.129P2-Gpr6

behavior/neurological phenotype

nervous system phenotype

References

Additional - Gpr6tm1Dgen related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Gpr6^tm1Dgen

Allele Symbol: Gpr6^tm1Dgen

Genotype: Gpr6^tm1Dgen/Gpr6^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6

Genotype: Gpr6^tm1Dgen/Gpr6^tm1Dgen
B6.129P2-Gpr6

Additional - Gpr6^tm1Dgen related