Ccrl2 knockout (KO) mice contain a neomycin cassette designed to disrupt the expression of Ccrl2 (chemokine (C-C motif) receptor-like 2). These mice may be useful for studying chemotaxis.
Ccrl2 (chemokine (C-C motif) receptor-like 2) encodes a chemokine receptor that facilitates leukocyte chemotaxis and is expressed on multiple cell types including astrocytes, B lymphocytes, dendritic cells, endothelial cells, macrophages, mast cells, microglia, and neutrophils. Ccrl2-deficient mice contain a neomycin cassette that disrupts expression of Ccrl2. Mice were created and characterized by Deltagen, Inc. Homozygotes are viable and fertile and exhibit no differences in aging, behavior, blood cell differentials,
body length, body mass, or serum chemistry as compared to wild-type. View phenotypic data developed by Deltagen.
The construct insert is reported to be "Neo555T." The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6NCrl and offspring were backcrossed to C57BL/6J for at least 7 generations.
|Allele Name||targeted mutation 1, Deltagen|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Ccrl2, chemokine (C-C motif) receptor-like 2|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A targeted mutation results in disruption of this gene. RT-PCR analysis detects a high level of gene transcript in all tissues tested.|
When maintaining a live colony, these mice are bred by homozygote sibling matings.
When using the Ccrl2-deficient mouse strain in a publication, please cite the originating article(s) and include JAX stock #005795 in your Materials and Methods section.