These mice carry an ENU-induced mutation and exhibit intermittent spasms, body tremors, and abnormal body movement.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.Read More +
The hind limbs of these mutants are kept close together, and show intense, intermittent spasms; body tremor during movement and rest also occurs intermittently, as does head tossing behavior. When lifting mutants into the air by their tail, the
hind limbs are drawn toward their body. Front limbs appear to be affected in some animals, i.e. are turned medially with apparent plantar flexion; these mutants move around slowly, are fragile and might not live to full maturity. The onset of this phenotype is at approximately 3 weeks of age (average 3.4+/-0.5 weeks of age; n=32). Standard pathology work-up on three mutants (18 - 26 days of age) revealed hypercellular Islets of Langerhans in the pancreas and wasting in the liver of one mutant; an atrophic thymus was noted in another mutant. No other abnormalities have been observed.
Mutants do not breed well, and a colony has to be maintained through ovarian transplants.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.
|Allele Name||neuroscience mutagenesis facility, 356|
|Allele Type||Chemically induced (ENU)|
|Gene Symbol and Name||nmf356, neuroscience mutagenesis facility, 356|
|Strain of Origin||C57BL/6J|
|Molecular Note||This phenotypic mutation was identified in an ENU mutagenesis screen.|