These mice carry a spontaneous mutation of the Grid2 gene and exhibit body tremors and abnormal gait.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.Read More +
The mutants show body tremor at rest and during movement, and intense trunk swaying, which can impair their ability to stand up-right on their hind limbs. They move around quite well, but draw their hind limbs high and close to their body when walking. This phenotype becomes visible at approximately 4 weeks of age (average 3.8 +/-0.53 weeks; n=41). Complementation analysis with Grid2ho-15J(Stock No. 005344; heterozygous x heterozygous matings) resulted in 11 affected mice in a total of 54 progeny demonstrating that nmf408 represents an allele of Grid2. Standard pathology work-up on one mutant (32 days of age) revealed no abnormalities. The mutants breed well and a colony can be maintained through regular breeding.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.
|Allele Name||hotfoot 17 Jackson|
|Allele Synonym(s)||Grid2dupE3; neuroscience mutagenesis facility, 408; NMF408|
|Gene Symbol and Name||Grid2, glutamate receptor, ionotropic, delta 2|
|Strain of Origin||C57BL/6J|
|Molecular Note||Exon 3 is duplicated.|