These Prkcq knock-out mice exhibit severely impaired T helper cell (Th2) immune responses as well as abnormal insulin signaling and glucose transport. They are suitable for use in applications related to T cell development, Th2-mediated disease, asthma, and diabetes.
Dr. Dan R. Littman, New York University Medical CenterRead More +
Mice homozygous for the targeted allele are viable, fertile, normal in size, and do not display any behavioral abnormalities. No endogenous or truncated protein product was detected in thymocytes or T cells. Mature T lymphocytes from null mice have blunted proliferative responses with decreased levels of both IL-2 and IL-2 receptor, and defective T cell receptor-initiated IkappaB-degradation/NF-kappaB activation. Homozygous mice exhibit severely impaired Th2, but normal Th1, immune responses as well as abnormal insulin signaling and glucose transport. Mutant mice also have defective regulatory T cell development (very low CD25 expression). This mutant may be suitable for use in studies related to T cell proliferation/signal transduction/immunodeficiency, Th2-mediated disease, asthma, and diabetes.
These mice have been found to also carry the Crb1rd8 allele.
A targeting vector replaced the endogenous exon coding for the ATP-binding site of the kinase domain (amino acids 396-451) with the neomycin resistance gene. The construct was electroporated into the 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric mice were bred to C57BL/6. Heterozygotes were backcrossed to C57BL/6 mice for 15 generations before being made homozygous.
|Allele Name||retinal degeneration 8|
|Allele Synonym(s)||Rd8-; nmf144|
|Gene Symbol and Name||Crb1, crumbs family member 1, photoreceptor morphogenesis associated|
|Gene Synonym(s)||7530426H14Rik; 7530426H14Rik; A930008G09Rik; A930008G09Rik; LCA8; RIKEN cDNA 7530426H14 gene; RIKEN cDNA A930008G09 gene; RP12|
|Strain of Origin||C57BL/6J|
|Molecular Note||The mutation in the rd8 mouse has been identified as a single base deletion in the Crb1 gene. This deletion causes a frame shift and a premature stop codon that truncates the transmembrane and cytoplasmic domain of the protein.|
|Allele Name||targeted mutation 1, Dan Littman|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||PKC-Theta-; PKCtheta-|
|Gene Symbol and Name||Prkcq, protein kinase C, theta|
|Gene Synonym(s)||A130035A12Rik; A130035A12Rik; AW494342; PKC theta; PKC-0; PKC-theta; PKCtheta; PRKCT; Pkcq; Pkcq; RIKEN cDNA A130035A12 gene; expressed sequence AW494342; nPKC-theta|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||The exon encoding the ATP-binding site of the kinase domain (aa 396 - 451) was replaced with a neo cassette inserted by homologous recombination. Protein was undetected in thymocytes or T cells obtained from mutant mice.|
|Mutations Made By|| |
Dr. Dan Littman, New York University Medical Center
When maintaining a live colony, these mice are maintained as homozygotes. Recommend specific pathogen free vivarium as homozygotes are T cell immunodeficient.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
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