These floxed mutant mice possess loxP sites flanking exons 3-5 of the Pik3c2b gene. This strain may be useful for generating conditional mutations in applications related to epidermal differentiation.
Paul Khavari, Stanford University
These mice possess loxP sites on either side of exons 3-5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain can be used to generate tissue-specific mutants of the floxed allele.
A loxP site flanked targeting vector containing neomycin and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 5 of the targeted gene, and another loxP site was inserted upstream of exon 3. The targeting vector was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 5 generations.
|Allele Name||targeted mutation 1, Paul A Khavari|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Pik3c2b, phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A loxP site was inserted upstream of exon 3 and a loxP-flanked neomycin selection cassette was inserted downstream of exon 5.|
|Mutations Made By|| |
Amy Truong, Stanford University
When maintaining a live colony, these mice are bred as homozygotes.
When using the C2Βfl/+ mouse strain in a publication, please cite the originating article(s) and include JAX stock #005702 in your Materials and Methods section.