These Lipg knock-out mice exhibit increased fasting plasma levels of total cholesterol, high-density lipoprotein (HDL) cholesterol, and phospholipids. They are suitable for use in cholesterol-related studies.
Thomas Quertermous, Stanford University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Lipg | lipase, endothelial |
Homozygous mice are viable and do not display any behavioral or histological defects. Although homozygotes are fertile, the donating investigator reports decreased fecundity. Northern blot analysis showed no endogenous gene expression in lung, liver, kidney, spleen, or aorta. Homozygotes have increased fasting plasma levels of total cholesterol, high-density lipoprotein (HDL) cholesterol, and phospholipids compared to wild type. Mutant mouse plasma apoA-I is increased while heparin-releasable phospholipase activity is impaired. Homozygous null mice have decreased arterial endothelium monocyte adhesion. This mutant can be used in studies of lipoprotein metabolism, atherosclerotic vascular disease, and other cholesterol-related studies.
A targeting vector containing a neomycin resistance gene was used to replace exon 1 of the endogenous gene. The construct was electroporated into 129S6/SvEv-derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. A resulting chimeric male was backcrossed to a C57BL/6J female. Heterozygous pups were bred to C57BL/6J for at least six generations before being interbred.
Allele Name | targeted mutation 1, Thomas Quertermous |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | LIPG- |
Gene Symbol and Name | Lipg, lipase, endothelial |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 18 |
Molecular Note | The gene was disrupted by insertion of a neomycin resistance cassette into exon 1 via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Northern blot analysis of lung, kidney, aorta, liver, and heart. |
Mutations Made By | Thomas Quertermous, Stanford University School of Medicine |
When maintaining a live colony, these mice can be bred as homozygotes, but heterozygous mice have greater fecundity.
When using the LIPG- mouse strain in a publication, please cite the originating article(s) and include JAX stock #005681 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Lipg<tm1Tq> |
Frozen Mouse Embryo | B6.129S-Lipg<tm1Tq>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S-Lipg<tm1Tq>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S-Lipg<tm1Tq>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S-Lipg<tm1Tq>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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