These Car3 knock-out mice exhibit shorter relaxation and half-relaxation times for single and tetanic twitches and a minor reduction tetanic force, but otherwise show no differences from wild type littermates. They are suitable for use in studies of the role of carbonic anhydrases.
Rod Levine, NIH-NHLBI
Mice homozygous for the targeted allele are viable, fertile, normal in size and life span and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected. Histological examination of tissues discerns no anomalies. Similarly, gas chromatography-mass spectrometry analysis of the fatty-acid distribution in serum, muscle, liver, and fat reveal no differences between mutant and wildtype mice. When exposed to an atmosphere of 100% oxygen homozygous mice display similar mean survival times as wildtype (~ 70 hours). The contractile properties of soleus muscle derived from mutant mice exhibit some differences from wildtype mice. Mutant mice display shorter relaxation and half-relaxation times for single and tetanic twitches and a minor reduction tetanic force. Microarray analysis detects an increase (~30%) in carbonic anhydrase 13 expression.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt 4.2kb of sequence encoding exons 3 to 5. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to 129S6/SvEvTac mice before being made homozygous.
|Allele Name||targeted mutation 1, Geumsoo Kim|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Car3, carbonic anhydrase 3|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||The targeting event replaced 4.2 kb of DNA containing exons 3, 4 and 5 with a neomycin resistance (PGK-neo ) cassette. Northern and western blot analyses verified that mRNA and protein encoded by the targeted gene are both absent in tissues of homozygous mutant mice. Microarray analysis revealed a small (~30% +/- 10%) but statistically significant increase in Car13 transcript levels in mice lacking CAR3.|
|Mutations Made By|| |
Geumsoo Kim, LB/NHLBI/NIH
When maintaining a live colony, these mice are bred as homozygotes.
When using the 129S6/SvEvTac-Car3tm1Gkim/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005358 in your Materials and Methods section.
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