These mice carry an ENU-induced mutation and exhibit ataxia.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.Read More +
The phenotype becomes visible at approximately 3 weeks of age (mean 3.5 +/- 0.7 weeks ; n=21). The hind limbs of mutants are set wide apart during movement and rest, the mice show intense body tremor with intermittent hind limb spasms, trunk swaying and forward staggering movements (cerebellar ataxia). They lose balance occasionally, which they regain quickly. When the mice are lifted up by their tail, the hind limbs tend to clutch to their body. Standard pathology work-up on two mutants (29 or 289 days of age) revealed a loss of granule cells in the cerebellum, which appeared less pronounced in the younger mouse. An over abundance of unmigrated granule cells located in the molecular layer was also noted in the older mouse. Brain histology, including Calbindin immunohistochemistry, was performed on two additional mutants (45 or 49 days of age) and confirmed the granule cell loss, but did not reveal any other abnormalities.
Female mutants (n/n) of this line breed well, males do not; a colony can be maintained through heterozygotes x heterozygotes matings.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.
|Allele Name||neuroscience mutagenesis facility, 313|
|Allele Type||Chemically induced (ENU)|
|Gene Symbol and Name||nmf313, neuroscience mutagenesis facility, 313|
|Strain of Origin||C57BL/6J|
|Molecular Note||This phenotypic mutant was identified in an ENU mutagenesis screen.|
Homozygous female mutants of this line breed well, males do not; a colony can be maintained through heterozygotes x heterozygotes matings.
When using the C57BL/6J-nmf313/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005298 in your Materials and Methods section.