These mice carry an ENU-induced mutation and are characterized by ataxic episodes.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Chemically induced (ENU) | Clcn1 | chloride channel, voltage-sensitive 1 |
The hind limbs of these mutants lose function intermittently, i.e. both or one or the other hind leg may lag behind, or may be stretched to the side or front during movement (average onset 4.6 +/- 0.8 weeks of age; n=15). These episodes usually last less than a minute, and the mice then move around quite normally. Front limbs appear to be affected to a lesser degree. When the mutants are lifted up by their tail, the hind legs come together and point down- or backwards. Because of the phenotypic similarity to that observed following mutations of the Clcn1 (Chloride channel 1) gene, complementation tests between NMF310 and Clcn1adr-mto were conducted. The results of heterozygous x heterozygous matings (NMF310 x Stock No. 001265 BALB/cByj-Clcn1adr-mto-2J/J), i.e. 6 affected mice in a total of 29 progeny, suggest that NMF310 represents an allele of Clcn1. Standard pathology work-up on one mutant (88 days of age) revealed no abnormalities. Mutants do not breed and the colony has to be maintained through ovarian transplants.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.
Allele Name | myotonia 6 Jackson |
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Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | Clcn1nmf310; neuroscience mutagenesis facility 310; NMF310 |
Gene Symbol and Name | Clcn1, chloride channel, voltage-sensitive 1 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 6 |
General Note | The hind limbs of these mutants appear to lose function intermittently, i.e. both or one or the other hind leg may lag behind, or may be stretched to the side or front during movement. These episodes usually last less than a minute, and the mice then move around quite normally. Front limbs appear to be affected to a lesser degree. When the mutants are lifted up by their tail, the hind legs come together and point down- or backwards. The mutants do not breed and the colony has to be maintained through ovarian transplants. |
Molecular Note | Complementation tests between NMF310 and Clcn1adr-mto were conducted. The results of heterozygous x heterozygous matings (NMF310 x BALB/cByj-Clcn1adr-mto2J), i.e. 6 affected mice in a total of 29 progeny, suggest that NMF310 represents an allele of Clcn1. |
When using the myotonia 6 Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #005253 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Unknown for Clcn1<adr-mto6J> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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