These floxed NR1 mice allow deletion of the GluN1 subunit of the N-methyl-D-aspartate receptor in Cre recombinase expressing cells/tissues. These mice may be useful in studying the NMDAR and its downstream signaling molecules/pathways (including alpha-CaMKII and mTOR), synaptic plasticity (learning and memory) and social behavior.
Dr. Susumu Tonegawa, Massachusetts Institute of Technology
These floxed NR1 (Grin1flox) mice possess loxP sites flanking approximately 12 kbp of sequence of the targeted gene that encodes the entire transmembrane domain and C-terminal region. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain expressing Cre recombinase in the CA3 region of the hippocampus (see Stock No. 006474 for example), this mutant mouse strain may be useful in studies of associative memory recall.
When bred to a strain expressing Cre recombinase in the CA1 region of the hippocampus (see Stock No. 005359 for example), this mutant mouse strain may be useful in studies of nonspacial memory.
A targeting vector containing the gene sequence, neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. A loxP site was inserted between exons 10 and 11. Another loxP site and a neomycin resistance gene were inserted at the 3' end of the gene, 3 kbp downstream of the last exon. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice. The colony was then backcrossed to C57BL/6J for eight generations prior to sending to The Jackson Laboratory Repository in 2005 (see SNP note below).
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
|Allele Name||targeted mutation 2, Susumu Tonegawa|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Flox-B; floxed-NR1; fNR1; Grin1fx; NR1flox|
|Gene Symbol and Name||Grin1, glutamate receptor, ionotropic, NMDA1 (zeta 1)|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Insertion of loxP sites flanking the entire transmembrane region (exons 11-21 through the 3' end of the gene).|
|Mutations Made By|| |
Dr. Susumu Tonegawa, Massachusetts Institute of Technology
When maintaining a live colony, these mice are bred as homozygotes.
When using the fNR1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #005246 in your Materials and Methods section.
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