These floxed mutant mice possess loxP sites flanking exon 2 of the Disp1 gene. This strain may be useful for generating conditional mutations in applications related to sonic hedgehog (Shh) signaling.
Andrew P McMahon, University of Southern California
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele, which is hypomorphic.
A targeting vector was used to insert loxP sites to flank exon 2. This construct was electroporated into 129X1/SvJ-derived AV3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting mice were then crossed to a mixed background. Additional information from the Donating Investigator is forthcoming.
|Allele Name||targeted mutation 2, Andrew P McMahon|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Disp1, dispatched RND transporter family member 1|
|Strain of Origin||129X1/SvJ|
|Molecular Note||A targeting vector was designed to flank exon 2, which encodes the amino terminal cytoplasmic domain and the first transmembrane domain, with loxP sites.|
|Mutations Made By|| |
Joe Vaughan, Harvard University
When maintained in a live colony, these mice are bred as heterozygotes on a mixed background.
When using the Disp1Δ2C mouse strain in a publication, please cite the originating article(s) and include JAX stock #005134 in your Materials and Methods section.