STOCK Disp1^tm2Amc/J

Stock number: 005134
Product name: Disp1^Δ2C
Research areas: Research Tools

Description

These floxed mutant mice possess loxP sites flanking exon 2 of the Disp1 gene. This strain may be useful for generating conditional mutations in applications related to sonic hedgehog (Shh) signaling.

Detailed description

These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele, which is hypomorphic.

Development

A targeting vector was used to insert loxP sites to flank exon 2. This construct was electroporated into 129X1/SvJ-derived AV3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting mice were then crossed to a mixed background. Additional information from the Donating Investigator is forthcoming.

Allele

Symbol: Disp1^tm2Amc
Name: targeted mutation 2, Andrew P McMahon
MGI accession ID: MGI:3512541
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Disp1
Marker name: dispatched RND transporter family member 1
Chromosome: 1
Strain of origin: 129X1/SvJ
Molecular note: A targeting vector was designed to flank exon 2, which encodes the amino terminal cytoplasmic domain and the first transmembrane domain, with loxP sites.