These mice carry an ENU-induced mutation characterized splayed hind limbs and difficulty remaining upright.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.Read More +
The mutants are small and have splayed hind limbs, which can be observed at approximately 3.6 weeks of age (mean 3.7+/-2.2 weeks; n=18). When moving around they have a tendency to lean and eventually fall over to the side, at times sliding for some distance, before they regain a walking position. Complementation analysis with B6.Cg-Rorasg/J (Stock Number 002651) has shown NMF267 to be an allele of Rora (RAR-related orphan receptor alpha), i.e. a heterozygote x heterozygote mating resulted in 2 mutants in a total of 9 progeny.
Standard pathology work-up on five mutants (22- 46 days of age) revealed a disorganized Purkinje cell layer and a loss of granule cells. These mutants are fragile, and a colony needs to be maintained through ovarian transplants.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.
|Allele Name||staggerer 3 Jackson|
|Allele Type||Chemically induced (ENU)|
|Allele Synonym(s)||neuroscience mutagenesis facility, 267; NMF267; Roranmf267|
|Gene Symbol and Name||Rora, RAR-related orphan receptor alpha|
|Strain of Origin||C57BL/6J|
|Molecular Note||This phenotypic mutation, identified in an ENU mutagenesis screen, was shown to be an allele of Rora by its failure to complement the original staggerer mutation.|