This knock-out mutant mouse may be useful in studies of asthma, allergic inflammation of the airway, inflammatory bowel disease, injury/trauma, G protein-coupled receptors, signal transduction, and a wide variety of immunological or inflammatory diseases.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
---|---|
N5+pN1F10
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | F2rl1 | coagulation factor II (thrombin) receptor-like 1 |
Homozygous mice are viable and fertile with no spontaneous abnormal phenotype. No endogenous gene expression is observed in liver, airway epithelium, smooth muscle, or vasculature of the lungs. Ovalbumin-induced allergic inflammation of the airway is significantly diminished in mutant mice, showing reduced eosinophil, neutrophil, and lymphocyte infiltration into bronchoalveolar lavage fluid. IgE levels in serum of ovalbumin sensitized homoyzgous mice are reduced 4-fold compared to wild-type. This targeted mutant mouse may be useful in studies of asthma, allergic inflammation of the airway, inflammatory bowel disease, injury/trauma, G protein-coupled receptors, signal transduction, and a wide variety of immunological or inflammatory diseases.
The targeted allele was generated by disrupting the endogenous locus with a 16 kb vector construct containing neomycin resistance and hygromycin genes. This vector was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were bred to "129SvJ" before being made homozygous. Homozygous mice were bred with transgenic mice carrying the human F2RL1 to obtain a double mutant mouse on a mixed FVB/N, C57BL/6, 129SvJ background. Upon its arrival at The Jackson Laboratory, the double mutant mouse was bred 5 generations to C57BL/6J and then simultaneously selected for the targeted allele and against the transgene. This strain no longer carries the human F2RL1 transgene.
Allele Name | targeted mutation 1, Mary Stevens |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | F2rl1tm1Nwb; PAR-2-; PAR2ko |
Gene Symbol and Name | F2rl1, coagulation factor II (thrombin) receptor-like 1 |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 13 |
Molecular Note | The endogenous locus was disrupted by the insertion of a construct containing hygromycin and neomycin selection genes. Transcript was undetected by Northern blot analysis of liver tissue obtained from homozygous mutant mice. |
Mutations Made By | Mary Stevens |
When maintaining a live colony, these mice may be bred as homozygotes.
When using the Par2ko mouse strain in a publication, please cite the originating article(s) and include JAX stock #004993 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for F2rl1<tm1Mslb> |
Frozen Mouse Embryo | B6.Cg-F2rl1<tm1Mslb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-F2rl1<tm1Mslb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-F2rl1<tm1Mslb>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-F2rl1<tm1Mslb>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.