B6.Cg-F2rl1^tm1Mslb/J

Stock number: 004993
Product name: Par2ko
Strain synonyms: PAR2ko
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

This knock-out mutant mouse may be useful in studies of asthma, allergic inflammation of the airway, inflammatory bowel disease, injury/trauma, G protein-coupled receptors, signal transduction, and a wide variety of immunological or inflammatory diseases.

Detailed description

Homozygous mice are viable and fertile with no spontaneous abnormal phenotype. No endogenous gene expression is observed in liver, airway epithelium, smooth muscle, or vasculature of the lungs. Ovalbumin-induced allergic inflammation of the airway is significantly diminished in mutant mice, showing reduced eosinophil, neutrophil, and lymphocyte infiltration into bronchoalveolar lavage fluid. IgE levels in serum of ovalbumin sensitized homoyzgous mice are reduced 4-fold compared to wild-type. This targeted mutant mouse may be useful in studies of asthma, allergic inflammation of the airway, inflammatory bowel disease, injury/trauma, G protein-coupled receptors, signal transduction, and a wide variety of immunological or inflammatory diseases.

Development

The targeted allele was generated by disrupting the endogenous locus with a 16 kb vector construct containing neomycin resistance and hygromycin genes. This vector was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were bred to "129SvJ" before being made homozygous. Homozygous mice were bred with transgenic mice carrying the human F2RL1 to obtain a double mutant mouse on a mixed FVB/N, C57BL/6, 129SvJ background. Upon its arrival at The Jackson Laboratory, the double mutant mouse was bred 5 generations to C57BL/6J and then simultaneously selected for the targeted allele and against the transgene. This strain no longer carries the human F2RL1 transgene.

Allele

Symbol: F2rl1^tm1Mslb
Name: targeted mutation 1, Mary Stevens
MGI accession ID: MGI:2389587
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: F2rl1
Marker name: coagulation factor II (thrombin) receptor-like 1
Chromosome: 13
Strain of origin: 129X1/SvJ
Molecular note: The endogenous locus was disrupted by the insertion of a construct containing hygromycin and neomycin selection genes. Transcript was undetected by Northern blot analysis of liver tissue obtained from homozygous mutant mice.