Fertility is compromised in Zfa knockout female mice, but it is unaffected in males.
Elizabeth M Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis of mutant testes. This mutant mouse strain may be useful in studies involving Y Chromosome gene targeting.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt 3kb of sequence encoding the entire targeted gene and approximately 1kb of flanking sequence. The construct was electroporated into 129P2/OlaHsd derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to 129P3/JEms (STOCK 002357) mice, and then backcrossed to C57BL/6J for 16 generations.
|Allele Name||targeted mutation 1, Elizabeth M Simpson|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Zfa-ps, zinc finger protein, autosomal, pseudogene|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||The entire gene was replaced with a PGK-neo cassette inserted by homologous recombination. The absence of transcript was confirmed by Northern blot analysis of homozygous mutant testes.|
|Mutations Made By|| |
Kathleen Banks, University of British Columbia
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6-Zfa KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004856 in your Materials and Methods section.