These double knock-out mutant mice have no mature T or B lymphocytes, no detectable NK cell cytotoxic activity, and lack serum immunoglobulin. They also develop thymic lymphomas.
Dr. Leonard D. Shultz, The Jackson Laboratory
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Prf1 | perforin 1 (pore forming protein) |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Rag1 | recombination activating gene 1 |
Mice that are homozygous for both targeted mutations are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities when housed under specific pathogen free conditions. These double homozygote mutant mice have no mature T or B lymphocytes, no detectable NK cell cytotoxic activity, and lack serum immunoglobulin. The number of nucleated spleen cells is significantly reduced in double mutant mice, when compared to the single homozygote, NOD.129S7(B6)-Rag1tm1Mom/J (Stock No. 003729). Although an increased number of DX5+CD122+ NK cells are found in the spleens of double mutants, these NK cells have impaired cytotoxic activity. The disruption of Prf1 ablates NK cell cytotoxic activity resulting in increased engraftment levels over that observed with Stock No. 003729. All mutant mice develop thymic lymphomas. This double mutant mouse strain may be useful in studies involving engraftment of human hematolymphoid cells.
Please see strain entries Stock No. 002407 and Stock No. 003729 for details regarding the construction of each mutant allele. This strain was produced by crossing female NOD.129S7(B6)-Rag1tm1Mom/J (Stock No. 003729) to male C57BL/6-Prf1tm1Sdz (Stock No. 002407). Resulting female progeny heterozygous for both mutant alleles were crossed to male NOD.129S7(B6)-Rag1tm1Mom/J (Stock No. 003729). Mice homozygous for the Rag1 mutant allele and heterozygous for the Prf1 allele were crossed to NOD.129S7(B6)-Rag1tm1Mom/J (Stock No. 003729) mice for 10 generations, and then intercrossed to produce double homozygotes.
Allele Name | targeted mutation 1, Sandoz Pharmaceuticals |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | P0; perf-; perforin-; perforin 0; Pfn-; pfp-; Pfptm1Sdz; pfpKO; pko; Prf-; Prf1-; Prf1tm/Sdz; prf1tm1 |
Gene Symbol and Name | Prf1, perforin 1 (pore forming protein) |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 10 |
Molecular Note | A neomycin selection cassette was inserted into exon 3. RT-PCR analysis on RNA derived from homozygous mice demonstrated that an abnormal transcript was produced from this allele. However, immunocytochemistry experiments on activated spleen cells derived from homozygous mice confirmed that no detectable protein was made from this allele. |
Mutations Made By | Dr. Birgit Lederman, University of Zurich |
Allele Name | targeted mutation 1, Peter Mombaerts |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Rag-; Rag1tm1Mom; RAG-1-; Rag1-; RAG1null; Rag-1KO |
Gene Symbol and Name | Rag1, recombination activating gene 1 |
Gene Synonym(s) | |
Site of Expression | expression is seen in bone marrow derived cell lines. |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 2 |
Molecular Note | A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
This strain is maintained as a homozygote for both mutant alleles, under specific pathogen free conditions.
When using the NOD/LtSz-Rag1nullPfpnull mouse strain in a publication, please cite the originating article(s) and include JAX stock #004848 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Rag1<tm1Mom> Homozygous for Prf1<tm1Sdz>, 1 pair minimum |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.