These Il10 knock-out mice exhibit type 1 diabetes development at the same rate as the NOD/Lt parental strain.
Dr. David Serreze, The Jackson Laboratory
Mice homozygous for the Il10tm1Cgn targeted mutation are viable and fertile when housed under SPF conditions. This mutant develops type 1 diabetes at the same rate as the NOD/Lt parental strain. The Il10 tm1Cgn mutation also renders this NOD/Lt stock susceptible to colitis (although not as severe as other strains of Il10 deficient mice) when maintained under standard housing conditions.
A targeting vector including a 24 bp DNA linker used to disrupt the first exon of Il10 after the fourth codon, a neomycin resistance cassette, and an additional termination codon in the third exon, was used for homologous recombination in E14-1 ES cells (129P2/OlaHsd-derived). Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were backcrossed to C57BL/6 females. The disrupted Il10 gene was then congenically transferred from the B6;129 segregating background to a C57BL/10 background in six generations. The mutation was then transferred to the NOD/Lt background using a marker assisted protocol. NOD/Lt mice heterozygous for this Il10tm1Cgn allele and homozygous for diabetes susceptibility loci (Idd) were intercrossed at N8 to develop mice homozygous for Il10tm1Cgn and all Idd loci.
|Allele Name||targeted mutation 1, University of Cologne|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||IL-10-; Il10-; IL-10 KO; IL-10KO; Il10tmCgn; IL-10KO|
|Gene Symbol and Name||Il10, interleukin 10|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A 500 bp genomic fragment containing codons 5-55 was replaced with a linker containing a termination codon followed by a neomycin cassette. A termination codon was also introduced into exon 3. No IL10 activity was detectable by ELISA assays in supernatants of in vitro cultures of Con A-stimulated splenic T cells derived from homozygous mice following infection with the nematode N. brasiliensis.|
|Mutations Made By|| |
Dr. Ralf Kuhn, Max-Delbrück Center for Molecular Medicine
When using the NOD.IL10null mouse strain in a publication, please cite the originating article(s) and include JAX stock #004266 in your Materials and Methods section.