Mice homozygous for the targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Ephx2 gene product (mRNA or protein) is detected. Systolic blood pressure is minimally effected in homozygous females but males exhibit markedly lower blood pressure. Both sexes also display an altered arachidonic acid metabolism; renal formation of dihydroxyeicosatrienoic acid is significantly diminished. This mutant mouse strain represents a model that may be useful in studies related to blood pressure regulation.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a region of the Ephx2 gene encoding exon 1. The construct was electroporated into 129X1/SvJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were mated to C57BL/6 mice.
|Allele Name||targeted mutation 1, Frank Gonzalez|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||sEH-; sEH-null|
|Gene Symbol and Name||Ephx2, epoxide hydrolase 2, cytoplasmic|
|Strain of Origin||129X1/SvJ|
|Molecular Note||Insertion of a neomycin resistance cassette into the translation initiation site in the first exon. Northern analysis did not detect transcript in liver from homozygous mutant mice. Immunoblot analysis did not detect protein in cytosolic fractions of liver from homozygous mutant mice.|
|Mutations Made By|| |
Frank Gonzalez, National Institutes of Health (NIH/NCI)
This strain originated on a B6;129X1 background and has been backcrossed to C57BL/6 for at least five generations.
When using the sEH KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004165 in your Materials and Methods section.