Il18-deficient mice exhibit reduced levels of interferon gamma in response to bacterial and lipopolysaccharide challenge, diminished natural killer cell activity, and impaired T helper lymphocyte response. These mice can be used to study the role of Il18 in inflammatory disorders.
Shizuo Akira, Osaka University
Genetic Background | Generation |
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N12F13
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Il18 | interleukin 18 |
Mice that are homozygous null for the Il18 gene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Il18 gene product (mRNA or protein) is detected. Homozygous null mice exhibit reduced levels of interferon gamma in response to heat killed bacteria and lipopolysaccharide. IL-12 levels in the serum are similar to wild type after LPS challenge, indicating that the decreased interferon gamma response in Il18 deficient mice is not due to low induction of IL-12. Il18 deficient mice also exhibit diminished natural killer cell activity and impaired T helper lymphocyte response.
A targeting vector containing neomycin resistance and Herpes simplex virus thymidine kinase genes was used to disrupt exons 3-5. The construct was transfected into 129P2/OlaHsd-derived E14-1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were backcrossed to C57BL/6 mice.
Allele Name | targeted mutation 1, Shizuo Akira |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | IL-18; IL-18-; Il18-; IL-18KO |
Gene Symbol and Name | Il18, interleukin 18 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | Replacement of exons 3, 4 and 5 with a neomycin resistance gene inserted in frame into exon 3. By Northern blot analysis, mRNA could not be detected in macrophages derived from homozygous mutant mice. By ELISA assay, protein cound not be detected in serum of homozygous mutant mice after intravenous injection of lipopolysaccharide. |
Mutations Made By | Shizuo Akira, Osaka University |
This strain originated on a B6;129P2 background and has been backcrossed to C57BL/6 for at least 8 generations(7/01). Coat color expected from breeding:Black
When using the Il18- mouse strain in a publication, please cite the originating article(s) and include JAX stock #004130 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Il18<tm1Aki> |
Frozen Mouse Embryo | B6.129P2-Il18<tm1Aki>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Il18<tm1Aki>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Il18<tm1Aki>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Il18<tm1Aki>/J Frozen Embryos | $3373.50 |
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