These mutant mice possess a floxed neomycin-resistance cassette inserted in the Mttp gene and a loxP site inserted upstream from the Mttp promoter. These mice may be useful in applications related to the study of microsomal triglyceride transfer protein and atherogenesis.
Dr. Stephen Young, UCLA
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Mttp | microsomal triglyceride transfer protein |
These mice possess loxP sites located 2.5 kb 5' of exon 1 and flanking a neomycin resistance gene inserted into intron 1 of the Mttp gene. Mice that are homozygous for this floxed Mttp allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain expressing interferon inducible Cre recombinase in liver tissue (see Stock No. 003556 for example), this mutant mouse strain may be useful in lipoprotein research.
A targeting vector containing Mttp sequence, neomycin resistance and herpes simplex virus thymidine kinase genes was utilized in the construction of this mutant. Similarly oriented loxP sites were placed 2.5 kb upstream of exon 1 and flanking the neomycin resistance gene (located in intron 1). The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Mice harboring the mutant allele were obtained and bred to homozygosity.
Allele Name | targeted mutation 2, Steven G Young |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Mttpfl |
Gene Symbol and Name | Mttp, microsomal triglyceride transfer protein |
Gene Synonym(s) | |
Site of Expression | cre mutation deletes floxed Mttp gene, reversing hypercholesterolemia phenotype |
Strain of Origin | 129S4/SvJae |
Chromosome | 3 |
Molecular Note | A neomycin resistance cassette flanked by loxP sites was inserted into intron 1. An additional loxP site was inserted 2.5 kb upstream of the promoter and exon 1. |
Mutations Made By | Dr. Stephen Young, UCLA |
This strain originated and is maintained as a homozygote on a B6;129S background.
When using the Mttpflox mouse strain in a publication, please cite the originating article(s) and include JAX stock #003902 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Mttp<tm2Sgy> |
Frozen Mouse Embryo | B6;129S-Mttp<tm2Sgy>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S-Mttp<tm2Sgy>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S-Mttp<tm2Sgy>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129S-Mttp<tm2Sgy>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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