B6;129S-Mttp^tm2Sgy/J

Stock number: 003902
Product name: Mttp^flox
Research areas: Metabolism Research, Research Tools

Description

These mutant mice possess a floxed neomycin-resistance cassette inserted in the Mttp gene and a loxP site inserted upstream from the Mttp promoter. These mice may be useful in applications related to the study of microsomal triglyceride transfer protein and atherogenesis.

Detailed description

These mice possess loxP sites located 2.5 kb 5' of exon 1 and flanking a neomycin resistance gene inserted into intron 1 of the Mttp gene. Mice that are homozygous for this floxed Mttp allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain expressing interferon inducible Cre recombinase in liver tissue (see Stock No. 003556 for example), this mutant mouse strain may be useful in lipoprotein research.

Development

A targeting vector containing Mttp sequence, neomycin resistance and herpes simplex virus thymidine kinase genes was utilized in the construction of this mutant. Similarly oriented loxP sites were placed 2.5 kb upstream of exon 1 and flanking the neomycin resistance gene (located in intron 1). The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Mice harboring the mutant allele were obtained and bred to homozygosity.

Allele

Symbol: Mttp^tm2Sgy
Name: targeted mutation 2, Steven G Young
MGI accession ID: MGI:2152202
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Mttp
Marker name: microsomal triglyceride transfer protein
Chromosome: 3
Strain of origin: 129S4/SvJae
Site of expression: cre mutation deletes floxed Mttp gene, reversing hypercholesterolemia phenotype
Molecular note: A neomycin resistance cassette flanked by loxP sites was inserted into intron 1. An additional loxP site was inserted 2.5 kb upstream of the promoter and exon 1.