B6;BKS-Atp2b2^dfw-3J/J

Stock number: 003892
Product name: dfw^3J
Research areas: Cell Biology Research, Neurobiology Research, Sensorineural Research

Description

These mice carry a spontaneous mutation at the Atp2b2 locus characterized by deafness and ataxia.

Detailed description

The spontaneous mutation Atp2b2^dfw-3J (or dfw^3J) contains a 4-nucleotide deletion in the third coding exon of the Atp2b2 gene. Atp2b2 transcript is undetectable by Western blot analysis of total brain protein. Homozygotes are deaf and ataxic. On the common CBA/CaJ background, another dfw null allele (Atp2b2^dfw-2J, Stock No. 002894) and the dfw^3J allele are indistinguishable in brain Atp2b2 mRNA and PMCA2 protein expression and in behavioral phenotype.

Development

The Atp2b2^dfw-3J mutation arose spontaneously in a C57BLKS/J-m+/+Lepr^db colony. Selection of non-obese mice as breeders eliminated Lepr^db from the colony. A single outcross to C57BL/6J was followed by test-crossing of F2 progeny to misty (m/m) mice to identify animals wild type at both Lepr and m from which to propagate Atp2b2^dfw-3J. Loss of Lepr^db was confirmed by the presence of only C57BL/6 microsatellite alleles in that region of Chr 4, which in C57BLKS is derived from DBA/2J.

Since the outcross to C57BL/6J, the strain has been inbred by crossing hosts of ovarian transplants from homozygous (Atp2b2^dfw-3J/Atp2b2^dfw-3J) females with +/? male siblings of the ovary donors, then mating the non-dwarf, obligate heterozygote (Atp2b2^dfw-3J/+) offspring among themselves.

Allele

Symbol: Atp2b2^dfw-3J
Name: deaf waddler 3 Jackson
MGI accession ID: MGI:1934613
Generation method: Spontaneous
Marker symbol: Atp2b2
Marker name: ATPase, Ca++ transporting, plasma membrane 2
Marker synonyms: D6Abb2e; DFNA82; Gena300; jog; PMCA2; PMCA2a; PMCA2i; Tmy; wms
Chromosome: 6
Strain of origin: BKS.Cg-Dock7^m +/+ Lepr^db/J
Molecular note: This mutation was determined to be allelic to the original deaf waddler mouse by complementation testing with Atp2b2^dfw.