The spontaneous mutation Atp2b2dfw-3J (or dfw3J) contains a 4-nucleotide deletion in the third coding exon of the Atp2b2 gene. Atp2b2 transcript is undetectable by Western blot analysis of total brain protein. Homozygotes are deaf and ataxic. On the common CBA/CaJ background, another dfw null allele (Atp2b2dfw-2J, Stock No. 002894) and the dfw3J allele are indistinguishable in brain Atp2b2 mRNA and PMCA2 protein expression and in behavioral phenotype.
The Atp2b2dfw-3J mutation arose spontaneously in a C57BLKS/J-m+/+Leprdb colony. Selection of non-obese mice as breeders eliminated Leprdb from the colony. A single outcross to C57BL/6J was followed by test-crossing of F2 progeny to misty (m/m) mice to identify animals wild type at both Lepr and m from which to propagate Atp2b2dfw-3J. Loss of Leprdb was confirmed by the presence of only C57BL/6 microsatellite alleles in that region of Chr 4, which in C57BLKS is derived from DBA/2J.
Since the outcross to C57BL/6J, the strain has been inbred by crossing hosts of ovarian transplants from homozygous (Atp2b2dfw-3J/Atp2b2dfw-3J) females with +/? male siblings of the ovary donors, then mating the non-dwarf, obligate heterozygote (Atp2b2dfw-3J/+) offspring among themselves.
|Allele Name||deaf waddler 3 Jackson|
|Gene Symbol and Name||Atp2b2, ATPase, Ca++ transporting, plasma membrane 2|
|Strain of Origin||BKS.Cg-Dock7m +/+ Leprdb/J|
|Molecular Note||This mutation was determined to be allelic to the original deaf waddler mouse by complementation testing with Atp2b2dfw.|
Homozygotes and heterozygotes are both poor breeders.
When using the dfw3J mouse strain in a publication, please cite the originating article(s) and include JAX stock #003892 in your Materials and Methods section.
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