Overview

Also Known As: EL-

These double knock-out mice exhibit a diminished response in neutrophil recruitment to the peritoneum in response to thioglycollate.

Donating Investigator

Dr. Richard Hynes, Massachusetts Institute of Technology

Genetic overview

Genetic Background	Generation

Sell^tm4Hyn

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Sell	selectin, lymphocyte

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Sele	selectin, endothelial cell

Research Applications

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice that are homozygous null for the Sele and Sell genes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Sele or Sell gene products (mRNA or protein) are detected. A slightly diminished response in neutrophil recruitment to the peritoneum in response to thioglycollate is observed, as is a diminished ability to interact with the venular endothelium resulting in increased "rolling" along the vessel wall. These mice are suitable for use in research applications studying leukocyte homeostasis, infectious diseases and inflammation.

Development

Sequential mutation by homologous recombination was utilized to replace the Sele and Sell genes with drug-resistance genes for hygromycin and puromycin (respectively) in 129S2/SvPas-derived D3 embryonic stem (ES) cells. This approach was necessary because the Sele and Sell genes are too closely linked for double-deficient animals to be generated by mating single-deficient animals. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric offspring obtained.

Genetics

Sell^tm4Hyn

Allele Symbol: Sell^tm4Hyn

Allele Name	targeted mutation 4, Richard Hynes
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Sell^tm4Hyn; targeted mutation 4, Richard Hynes
Gene Symbol and Name	Sell, selectin, lymphocyte
Gene Synonym(s)	AI528707; A.11; CD62L; L-selectin; LECAM-1; LNHR; LEU8; Lyam1; lymph node homing receptor; lymphocyte adhesion molecule 1; lymphocyte antigen 22; PLNHR; lymphocyte antigen m22; Lyam-1; Lnhr; Ly-22; Ly-m22; Lyam-1; expressed sequence AI528707; LECAM1; LSEL; LAM1; Ly-m22; LYAM1; Ly-22; TQ1; Lnhr; Lyam1
Strain of Origin	129S2/SvPas
Chromosome	1
Molecular Note	The three selectin genes, Sele, Sell, and Selp, are found within a 300 kb genomic region. To generate double knockouts, ES cells heterozygote for the Selp^tm1Hyn and Sele^tm2Hyn null alleles in trans to each other were targeted a third time. A PGK-puro cassette was used to delete a 2.1 kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. Flow cytometric analysis of homozygote leukocytes showed an absence of the L-selectin and E-selectin protein while P-selectin express was unaffected. Thus, this targeted mutation occurred in cis with the Sele^tm2Hyn allele and in trans with the Selp^tm1Hyn allele. Due to their genomic proximity, the Sell^tm3Hyn and Sele^tm2Hyn alleles should propagate together through the offspring.

Sele^tm2Hyn

Allele Symbol: Sele^tm2Hyn

Allele Name	targeted mutation 2, Richard Hynes
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Sele^tm2Hyn; targeted mutation 2, Richard Hynes
Gene Symbol and Name	Sele, selectin, endothelial cell
Gene Synonym(s)	Elam; LECAM2; CD62E; endothelial adhesion molecule; E-selectin; ELAM; ELAM1; ESEL; Elam
Strain of Origin	129S2/SvPas
Chromosome	1
Molecular Note	A modified D3 ES cell line heterozygous for the Selp^tm1Hyn allele was targeted a second time. The exons encoding the signal peptide, lectin domain, and a portion of the epidermal growth factor domain were replaced by the insertion of a PGK-hygro cassette. Both Northern and RT-PCR analysis revealed an absence of normal mRNA for E-selectin but the presence of mRNA for P-selectin in extracts from cardiac and pulmonary tissue of homozygous mutant mice. This targeted mutation occurred in trans with the Selp^tm1Hyn mutation and were subsequently bred apart.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Sele^tm2Hyn related

Additional - Sell^tm4Hyn related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: Sell^tm1Hyn
- Standard PCR: Sele^tm1Hyn
- Genotyping resources and troubleshooting
Breeding Considerations

This strain originated on a B6;129S2 background and is maintained as a homozygote. The donating lab maintained these mice by avoiding brother-sister matings.
- Additional Breeding and Husbandry Support
Citation
When using the EL- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003806 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Homozygous for Sele<tm1Hyn>, Homozygous for Sell<tm1Hyn>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Also Known As: EL-

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Selltm4Hyn

Allele Symbol: Selltm4Hyn

Seletm2Hyn

Allele Symbol: Seletm2Hyn

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Seletm2Hyn related

Additional - Selltm4Hyn related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Sell^tm4Hyn

Allele Symbol: Sell^tm4Hyn

Sele^tm2Hyn

Allele Symbol: Sele^tm2Hyn

Additional - Sele^tm2Hyn related

Additional - Sell^tm4Hyn related