Homozygous Dab1-null mice become tremulous and ataxic at approximately postnatal day 10. Multiple defects can be detected in brain tissue. Lamellar structures in the cortex and hippocampus appear disorganized and the cerebellum is small and disorganized. Although homozygotes null reportedly die at 4-5 weeks on a mixed B6,129 or 129/Sv background, mice on a BALB/cByJ are viable. Homozygous females may breed but tend to be poor mothers.
|Allele Name||targeted mutation 1, Jonathan A Cooper|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Dab1-; Dab1 ko; mdab1-1|
|Gene Symbol and Name||Dab1, disabled 1|
|Strain of Origin||129S4/SvJaeSor|
|Molecular Note||A neomycin resistance cassette replaced a genomic fragment containing sequences encoding the first 47 amino acids of the PTB domain. Western blot analysis on brain lysates derived from homozygous mice demonstrated the absence of the protein.|
|Mutations Made By|| |
Jonathan Cooper, Fred Hutchinson Cancer Research Center
When maintaining a live colony, heterozygous mice may be bred to wildtype siblings, or to BALB/cByJ inbred mice (Stock No. 001026). Homozygous females may breed but tend to be poor mothers. The expected coat color from breeding is albino.
When using the mdab1-1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003581 in your Materials and Methods section.