CBy.129S4-Dab1^tm1Cpr/J

Stock number: 003581
Product name: mdab1-1
Research areas: Developmental Biology Research, Neurobiology Research

Description

These Dab1 knock-out mice exhibit ataxia and have multiple defects in brain tissue.

Detailed description

Homozygous Dab1-null mice become tremulous and ataxic at approximately postnatal day 10. Multiple defects can be detected in brain tissue. Lamellar structures in the cortex and hippocampus appear disorganized and the cerebellum is small and disorganized. Although homozygotes null reportedly die at 4-5 weeks on a mixed B6,129 or 129/Sv background, mice on a BALB/cByJ are viable. Homozygous females may breed but tend to be poor mothers.

Allele

Symbol: Dab1^tm1Cpr
Name: targeted mutation 1, Jonathan A Cooper
MGI accession ID: MGI:1861918
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Dab1
Marker name: disabled 1
Chromosome: 4
Strain of origin: 129S4/SvJaeSor
Molecular note: A neomycin resistance cassette replaced a genomic fragment containing sequences encoding the first 47 amino acids of the PTB domain. Western blot analysis on brain lysates derived from homozygous mice demonstrated the absence of the protein.