BALB/c-Il4ra^tm1Sz/J

Stock number: 003514
Product name: Il4rα KO
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research

Description

Homozygous Il4ra knockout mice show no overt phenotypic abnormalities, but exhibit a loss of IL4-mediated signal transduction.

Detailed description

Homozygous Il4ra (interleukin 4 receptor, alpha) knockout mice show no overt phenotypic abnormalities, but exhibit a loss of IL4 signal transduction. This results in a diminished TH2 helper T cell response to helminthic parasite infections. These mutant mice provide a tool for analyzing the role of IL4/IL4RA in immunological pathways, including those that prime TH2 responses in infectious disease and other pathologies.

Development

Exons 7, 8, and 9 were replaced with a PGKneomycin resistance cassette inserted in reverse transcriptional orientation. The mutation was created through homologous recombination in BALB/cJ-derived BALB/c-I embryonic stem (ES) cells. Resultant chimeric males were bred to BALB/cJ females once before sibling matings were set up by the donating laboratory.

Allele

Symbol: Il4ra^tm1Sz
Name: targeted mutation 1, Leonard Shultz
MGI accession ID: MGI:1861951
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il4ra
Marker name: interleukin 4 receptor, alpha
Chromosome: 7
Strain of origin: BALB/cJ
Molecular note: A neomycin resistance cassette replaced exons 7 - 9 of the gene.