Homozygous Il4ra knockout mice show no overt phenotypic abnormalities, but exhibit a loss of IL4-mediated signal transduction.
Dr. Leonard D. Shultz, The Jackson Laboratory
Genetic Background | Generation |
---|---|
F?+F48
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Il4ra | interleukin 4 receptor, alpha |
Homozygous Il4ra (interleukin 4 receptor, alpha) knockout mice show no overt phenotypic abnormalities, but exhibit a loss of IL4 signal transduction. This results in a diminished TH2 helper T cell response to helminthic parasite infections. These mutant mice provide a tool for analyzing the role of IL4/IL4RA in immunological pathways, including those that prime TH2 responses in infectious disease and other pathologies.
Exons 7, 8, and 9 were replaced with a PGKneomycin resistance cassette inserted in reverse transcriptional orientation. The mutation was created through homologous recombination in BALB/cJ-derived BALB/c-I embryonic stem (ES) cells. Resultant chimeric males were bred to BALB/cJ females once before sibling matings were set up by the donating laboratory.
Allele Name | targeted mutation 1, Leonard Shultz |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | IL-4R-; IL-4R KO; Il4ratm1; IL-4Ralpha-; Il-4ralphatm1Sz |
Gene Symbol and Name | Il4ra, interleukin 4 receptor, alpha |
Gene Synonym(s) | |
Strain of Origin | BALB/cJ |
Chromosome | 7 |
Molecular Note | A neomycin resistance cassette replaced exons 7 - 9 of the gene. |
Mutations Made By | Dr. Leonard Shultz, The Jackson Laboratory |
When using the Il4rα KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003514 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Il4ra<tm1Sz> |
Frozen Mouse Embryo | BALB/c-Il4ra<tm1Sz>/J | $2595.00 |
Frozen Mouse Embryo | BALB/c-Il4ra<tm1Sz>/J | $2595.00 |
Frozen Mouse Embryo | BALB/c-Il4ra<tm1Sz>/J | $3373.50 |
Frozen Mouse Embryo | BALB/c-Il4ra<tm1Sz>/J | $3373.50 |
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