Mice homozygous for the targeted mutation do not have MHC class II molecules on the surface of splenic B cells, dendritic cells, or on peritoneal macrophages and somatic tissues stimulated by interferon. Therefore they have deficient T-dependent antigen responses and MHC class II-mediated allogeneic responses.
Dr. Richard A. Flavell, Yale University School of Medicine
Mice homozygous for the targeted mutation are viable and fertile when housed under specific pathogen-free conditions. Mice do not express conventional MHC class II molecules on the surface of splenic B cells and dendritic cells. In addition, interferon gamma stimulated peritoneal macrophages and somatic tissues from homozygous mutant mice do not express MHC class II molecules. The levels of invarient chain and H2m gene transcripts are substantially decreased in class II transactivator deficient mice. Homozygous mice have very few mature CD4 T cells in the periphery despite MHC class II expression in the thymus.
|Allele Name||targeted mutation 1, Cheong-Hee Chang|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||C2tatm1Ccum; CIITA-; CIITA C-; MHC classII-|
|Gene Symbol and Name||Ciita, class II transactivator|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A genomic fragment containing exons which encode critical regions of the protein was replaced with a neomycin selection gene. Northern blot analysis on RNA derived from homozygous mice demonstrated that no detectable protein was produced from this allele.|
|Mutations Made By|| |
Dr. Cheong-Hee Chang, Indiana University