B6.129S2-Ciita^tm1Ccum/J

Stock number: 003239
Product name: CIITA-
Research areas: Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools

Description

Mice homozygous for the targeted mutation do not have MHC class II molecules on the surface of splenic B cells, dendritic cells, or on peritoneal macrophages and somatic tissues stimulated by interferon. Therefore they have deficient T-dependent antigen responses and MHC class II-mediated allogeneic responses.

Detailed description

Mice homozygous for the targeted mutation are viable and fertile when housed under specific pathogen-free conditions. Mice do not express conventional MHC class II molecules on the surface of splenic B cells and dendritic cells. In addition, interferon gamma stimulated peritoneal macrophages and somatic tissues from homozygous mutant mice do not express MHC class II molecules. The levels of invarient chain and H2^m gene transcripts are substantially decreased in class II transactivator deficient mice. Homozygous mice have very few mature CD4 T cells in the periphery despite MHC class II expression in the thymus.

Allele

Symbol: Ciita^tm1Ccum
Name: targeted mutation 1, Cheong-Hee Chang
MGI accession ID: MGI:1857456
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ciita
Marker name: class II transactivator
Chromosome: 16
Strain of origin: 129S2/SvPas
Molecular note: A genomic fragment containing exons which encode critical regions of the protein was replaced with a neomycin selection gene. Northern blot analysis on RNA derived from homozygous mice demonstrated that no detectable protein was produced from this allele.