The spontaneous mutation carried in these mice consists of a large deletion spanning about a 46.7 kb interval that includes the entire sequences of Lrrc75b (AI646023) and Ggt1 and the first exon and part of the first intron of Ggt5.
Ann Davis, Bayer Corporation
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Del(Lrrc75b-Ggt5)1Bayer | deletion Chr 10, Bayer 1 |
The dwarf-Bayer mutation (dwgBayer) occurred spontaneously in an embryonic stem cell line during genetic targeting of the insulin receptor gene. The phenotype of the mice resemble drawf grey (dwg, Stock No. 001743) and subsequent characterization determined that the two mutations are allelic. Homozygous mutant mice are grey and smaller in size than wildtype littermates.
This multigenic deletion occurred in a 129/SvEv ES cell line at Bayer Pharmaceutical Division in West Haven, Connecticut prior to 1995. The ES cell was used for a targeted disruption of a gene on Chromosome 8 and the chimeras generated did not display the dwarf gray phenotype. Four male chimeras were bred to both Black Swiss mice and 129/SvEv females and all of the offspring appeared normal, but when intercrossed the dwarf gray phenotype appeared in the F1 offspring for all four lines, indicating that the mutation derived from the ES cell line. In early 1996 mice at generation F2 or F3 on the 129/SvEv background, with no Black Swiss, were sent to The Jackson Laboratory and bred to C3H/HeSnJ for re-derivation then maintained by sibling inbreeding. The targeted disruption on Chromosome 8 is absent from this line. In 2003 embryos were generated for cryopreservations via in vitro fertilization of heterozygous males and either heterozygous or wildtype females, which were at generation N1F15.
Allele Name | deletion Chr 10, Bayer 1 |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | Del(10AI646023-Ggt5)1Bayer; dwarf grey Bayer; dwgBayer; Ggt1dwg-Bayer |
Gene Symbol and Name | Del(Lrrc75b-Ggt5)1Bayer, deletion Chr 10, Bayer 1 |
Gene Synonym(s) | |
Strain of Origin | 129S/SvEv |
Chromosome | 10 |
Molecular Note | This mutation arose spontaneously in an ES cell line during a targeted mutagenesis experiment for an unrelated gene (insulin receptor). This mutation consists of a large deletion spanning about a 46.7 kb interval that includes the entire sequences of AI646023 and Ggt1 and the first exon and part of the first intron of Ggt5. |
When using the dwgBayer mouse strain in a publication, please cite the originating article(s) and include JAX stock #003100 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Homozygous or wildtype for dwg<Bayer>, 1 pair minimum |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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