B6;129S-Apob^tm1Sgy/J

Stock number: 002876
Product name: apo-B⁴⁸
Research areas: Cardiovascular Research, Mouse/Human Gene Homologs

Description

These Apob knock-out mice exhibit low LDL cholesterol.

Detailed description

Mice homozygous for the Apob ^tm1Sgy targeted mutation are viable and fertile. They show normal expression of APOB48 in the gut but have no expression of APOB100. Homozygous mice show no developmental defects indicating that APOB100 synthesis in the yolk sac is not necessary for normal development. Homozygotes have lower serum LDL cholesterol levels compared to wildtype and APOB100 expressing mice (B6,129-Apob ^tm2Sgy, Stock No. 002877).

Allele

Symbol: Apob^tm1Sgy
Name: targeted mutation 1, Stephen G Young
MGI accession ID: MGI:1857303
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Apob
Marker name: apolipoprotein B
Chromosome: 12
Strain of origin: 129S7/SvEvBrd-Hprt⁺
Molecular note: A "hit and run"-type vector was used to create a stop signal in codon 2179 (codon 2153 in the original publication) in sequences corresponding to the apo-B48 editing codon. Western blot analysis on plasma derived from heterozygous and homozygous mice demostrated that the expression of the ApoB48 isoform is unaffected by this mutation, while no ApoB100 isoform is produced from this allele.
General note: Levels of LDL-cholesterol, VLDL, and triglyceride LDL are lower relative to wild-type mice in APOB48 only mice in the presence of wild-type APOE production(J:33830). APOB48 only mice have higher levels of cholesterol and more atherosclerotic lesions than APOB100 only mice in the absence of APOE (J:41510).