At birth, mice homozygous for the targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No transcript is detected in splenocytes or peritoneal macrophages. Thioglycollate-elicited peritoneal macrophages from homozygotes exhibit a delayed period before reaching peak mobilization (72 hours vs. 36 for wild type mice).
A targeting vector containing a neomycin resistance gene was used to disrupt the single coding exon of the targeted gene. The construct was electroporated into 129P2/OlaHsd-derived E14TG2A embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Correctly targeted ES cells were injected into C57BL/6J blastocysts to obtain chimeric animals.
|Allele Name||targeted mutation 1, William A Kuziel|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CCR5-; CCR5 KO; Cmkbr5tm1Kuz|
|Gene Symbol and Name||Ccr5, chemokine (C-C motif) receptor 5|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||The exon containing the entire coding region was replaced with a neomycin resistance gene inserted by homologous recombination.|
|Mutations Made By|| |
Dr. William Kuziel, Protein Design Laboratories
The strain is maintained by mating homozygous siblings. Expected coat color from breeding:Black
When using the CCR5- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002782 in your Materials and Methods section.