B6;129P2-Ccr5^tm1Kuz/J

Stock number: 002782
Product name: CCR5-
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research

Description

These Ccr5 knock-out mice exhibit delayed mobilization of peritoneal macrophages.

Detailed description

At birth, mice homozygous for the targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No transcript is detected in splenocytes or peritoneal macrophages. Thioglycollate-elicited peritoneal macrophages from homozygotes exhibit a delayed period before reaching peak mobilization (72 hours vs. 36 for wild type mice).

Development

A targeting vector containing a neomycin resistance gene was used to disrupt the single coding exon of the targeted gene. The construct was electroporated into 129P2/OlaHsd-derived E14TG2A embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Correctly targeted ES cells were injected into C57BL/6J blastocysts to obtain chimeric animals.

Allele

Symbol: Ccr5^tm1Kuz
Name: targeted mutation 1, William A Kuziel
MGI accession ID: MGI:1861911
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ccr5
Marker name: chemokine (C-C motif) receptor 5
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Molecular note: The exon containing the entire coding region was replaced with a neomycin resistance gene inserted by homologous recombination.