These Plau knock-out mice exhibit occasional fibrin deposition and excessive fibrin deposition when combined with the Plat knock-out. They are suitable for use in applications related to the study of the fibrinolytic system.
Dr. Peter Carmeliet, University of Leuven
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Plau | plasminogen activator, urokinase |
Homozygotes develop normally, are fertile and have a normal life span. Rectal prolapse of a non-infectious origin develops in 9% of homozygotes and/or extensive non-healing ulcerations occur at the eyelids and around the face. Small, focal fibrin deposits are occasionally seen in the intestines and in the sinusoids of the liver, and excessive fibrin deposits are seen in ulcerated skin or prolapsed rectum. Pulmonary clot lysis is comparable to that seen in normal wildtype siblings. Endotoxin induced venous thrombosis is increased over normal wildtype siblings. Fibrin dissolution by PLAU-deficient macrophages is greatly reduced but macrophage invasion into the peritoneal cavity after thioglycollate injection is unaffected. Homozygous knockout mice have increased levels of Abeta42 and Abeta40 in plasma. Brain Abeta levels are not significantly different than controls. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Allele Name | targeted mutation 1, Richard C Mulligan |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | UPA-; u-PA- |
Gene Symbol and Name | Plau, plasminogen activator, urokinase |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 14 |
Molecular Note | The gene was disrupted using neomycin resistance cassette. The vector replaced genomic sequences encompassing all but 23 amino acids of the coding sequence. Targeting was confirmed by the absence of gene specific mRNA and immunoreactivity. |
Mutations Made By | Dr. Peter Carmeliet, University of Leuven |
This strain can be maintained through homozygous sibling matings.
When using the u-PA- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002329 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Plau<tm1Mlg> |
Frozen Mouse Embryo | FVB.129S2-Plau<tm1Mlg>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB.129S2-Plau<tm1Mlg>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB.129S2-Plau<tm1Mlg>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB.129S2-Plau<tm1Mlg>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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