B6 x STOCK In(10)17Rk/J

Stock number: 001962
Product name: pygmy Jackson

Description

This strain carries a chromosomal inversion and is characterized by small size and sterility.

Detailed description

This triethylene melamine-induced inversion of approximately 60 cM has its proximal breakpoint in intron 2 of Txlnb and its distal breakpoint in intron 3 of Hmga2. A fusion transcript of Hmg2a exons 4 and 5 and Txlnb exons 1 and 2 was identified in embryonic day 12.5 samples. Homozygotes have a pygmy phenotype, initially designated pygmy Jackson, which was proven allelic by comlementation test with Hmg2a^pg. These homozygotes are small and sterile. Although heterozygotes have reduced levels of Tlxnb in heart and femur muscle and homozygotes lack wildtype Txlnb transcript, which is usually expressed specifically in muscle, a muscle phenotype has not been found.

Development

DBA/2J males were treated with .3mg/kg body weight triethylene melamine and these treated males were bred with C57BL/6J females. In(10)17Rk was found in the offspring. This mutation was maintained by Tom Roderick and the Kitl^Sl mutation was crossed into the stock as a repulsion marker for maintenance. Embryos were cryopreserved from C57BL/6J females bred with In(10)17Rk +/+ Kitl^Sl repulsion males. Steel colored cryo-recovered pups will not have the inversion while non-steel pups will.

Allele

Symbol: In(10)17Rk
Name: inversion, Chr 10, Roderick 17
MGI accession ID: MGI:3790439
Generation method: Chemically induced (other)
Marker symbol: In(10)17Rk
Marker name: inversion, Chr 10, Roderick 17
Chromosome: 10
Strain of origin: DBA/2J
Molecular note: This triethylene melamine-induced inversion of approximately 60 cM causes a homozygous pygmy phenotype, it is allelic by complementation test with Hmga2^pg, the distal breakpoint is in intron 3 of Hmga2, and the proximal breakpoint is in intron 2 of Txlnb. A 3.7 kb fusion transcript of Hmga2 exons 4 and 5 and Txlnb exons 1 and 2 is produced. No other Txlnb transcript was found by northern blot of homozygous heart or femur.

Allele

MGI accession ID: MGI:2671945
Marker symbol: Txlnb
Marker name: taxilin beta
Chromosome: 10

Allele

MGI accession ID: MGI:104055
Marker symbol: In(10)17Rk-p
Marker name: inversion, Chr 10, Roderick 17, proximal
Chromosome: 10

Allele

MGI accession ID: MGI:1333490
Marker symbol: In(10)17Rk-d
Marker name: inversion, Chr 10, Roderick 17, distal
Chromosome: 10

Allele

MGI accession ID: MGI:101761
Marker symbol: Hmga2
Marker name: high mobility group AT-hook 2
Chromosome: 10