STOCK Tg(Fabp1-GH1)10Bir/J

Stock number: 001788
Research areas: Cell Biology Research, Developmental Biology Research, Endocrine Deficiency Research, Internal/Organ Research, Research Tools

Detailed description

These L-FAB^{-596 to +21}/hGH mice express a transgene containing a human growth hormone gene whose 5' regulatory elements have been replaced by nucleotides -596 to +21 from the rat liver fatty-acid binding protein gene. Expression of hGH expression is observed in enterocytes and hepatocytes; however, anomalous expression is observed in small intestinal crypts, colon, and renal proximal tubular epithelial cells.

Allele

Symbol: Tg(Fabp1-GH1)10Bir
Name: transgene insertion 10, Edward H Birkenmeier
MGI accession ID: MGI:3800339
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Fabp1-GH1)10Bir
Marker name: transgene insertion 10, Edward H Birkenmeier
Chromosome: UN
Strain of origin: (C57BL/6J x LT/Sv)F1
Expressed genes: GH1,growth hormone 1,human
Molecular note: This transgene comprises a human growth hormone gene whose 5' regulatory elements have been replaced by nucleotides -596 to +21 from the rat liver fatty-acid binding protein gene. Mice of this line (from founder G0#19) have ~800 transgene copies per haploid genome, as measured by densitometric analysis of Southern blots. Expression of human growth hormone mRNA, determined by dot-blot analysis, correlates generally with that of endogenous mouse Fabp1 mRNA in liver and intestine, including a proximal-to-distal declining expression gradient in the small intestine (with somewhat higher hGH expression in the ileum); neither is expressed in several other tissues examined, but hGH, unlike Fabp1, is expressed in cecum, colon and kidneys of transgenic mice. This "ectopic" expression is suppressed by more 5' regulatory sequences present in similar transgenes with a longer Fabp1 promoter (See Tg(Fabp1-GH1)5Bir).