These L-FAB-596 to +21/hGH mice express a transgene containing a human growth hormone gene whose 5' regulatory elements have been replaced by nucleotides -596 to +21 from the rat liver fatty-acid binding protein gene. Expression of hGH expression is observed in enterocytes and hepatocytes; however, anomalous expression is observed in small intestinal crypts, colon, and renal proximal tubular epithelial cells.
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
These L-FAB-596 to +21/hGH mice express a transgene containing a human growth hormone gene whose 5' regulatory elements have been replaced by nucleotides -596 to +21 from the rat liver fatty-acid binding protein gene. Expression of hGH expression is observed in enterocytes and hepatocytes; however, anomalous expression is observed in small intestinal crypts, colon, and renal proximal tubular epithelial cells.
Expressed Gene | GH1, growth hormone 1, human |
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Site of Expression |
Allele Name | transgene insertion 10, Edward H Birkenmeier |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | L-FABP(-596 to +21)/hGH transgene; short promoter L-FABP-hGH transgene; Tg(L-FABP, S-GH)10Bir |
Gene Symbol and Name | Tg(Fabp1-GH1)10Bir, transgene insertion 10, Edward H Birkenmeier |
Gene Synonym(s) | |
Promoter | Fabp1, fatty acid binding protein 1, rat |
Expressed Gene | GH1, growth hormone 1, human |
Strain of Origin | (C57BL/6J x LT/Sv)F1 |
Chromosome | UN |
Molecular Note | This transgene comprises a human growth hormone gene whose 5' regulatory elements have been replaced by nucleotides -596 to +21 from the rat liver fatty-acid binding protein gene. Mice of this line (from founder G0#19) have ~800 transgene copies per haploid genome, as measured by densitometric analysis of Southern blots. Expression of human growth hormone mRNA, determined by dot-blot analysis, correlates generally with that of endogenous mouse Fabp1 mRNA in liver and intestine, including a proximal-to-distal declining expression gradient in the small intestine (with somewhat higher hGH expression in the ileum); neither is expressed in several other tissues examined, but hGH, unlike Fabp1, is expressed in cecum, colon and kidneys of transgenic mice. This "ectopic" expression is suppressed by more 5' regulatory sequences present in similar transgenes with a longer Fabp1 promoter (See Tg(Fabp1-GH1)5Bir). |
When using the STOCK Tg(Fabp1-GH1)10Bir/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #001788 in your Materials and Methods section.
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