Mice homozygous for the dwarf grey spontaneous mutation (Gtg1dwg) are viable but do not breed. Homozygous mutant mice have grey coats, are smaller than littermates, and develop cataracts by 3-4 weeks of age. Additional characteristics include increased numbers of osteoclasts, reduced bone mass, reduced red pulp area of the spleen, and slightly reduced thyroxin levels.
|Allele Name||dwarf grey|
|Gene Symbol and Name||Ggt1, gamma-glutamyltransferase 1|
|Strain of Origin||(STOCK Pou1f1dw x T(8;16)17H)F1|
|Molecular Note||A 13 bp deletion is present in exon 7. RT-PCR analysis detected 4 different transcripts in homozygous mice, the expected 13 bp deletion (type 1), a 75 bp deleted version that includes the 13 bp deletion (type 2), a version lacking all of exon 3 (type 3) and a version lacking exons 3 and 4 (type 4) both also having the 13 bp deletion in exon 7. Some clones of types 1, 3 , and 4 also include partial or complete retention of introns 9 or 10. The type 1, 3, and 4 transcripts are predicted to result in a frameshift and premature termination at amino acid residue 281, 57 , and 63, respectively. The type 2 transcript is predicted to create a 25 amino acid deletion spanning residues 270 - 294.|
When using the dwarf grey mouse strain in a publication, please cite the originating article(s) and include JAX stock #001743 in your Materials and Methods section.