This stock carries a triethylene melamine induced lethal mutation made from mice carrying the In(1)1Rk inversion (Stock No. 000716).
The In(1)1Rk inversion was generated in an irradiated DBA/2J male which was then bred to C57BL/6J. The In(7)13Rk inversion was generated in a triethylene melamine treated C3D2F1 male which was then bred to a C57BL/6J female. Males doubly homozygous for both In(1)1Rk and In(7)13Rk were treated with 0.3 mg/kg triethylene melamine and were then bred to linkage testing stock females with Pax3Sp Mlphln/+ Mlphln on Chromosomes 1 and Gpib Hbbs/Gpib Hbbs on Chromosomes 7. The In(1)1Rk + +/+ Pax3Sp Mlphln, In(7)13Rk Gpia Hbbd/+ Gpib Hbbs offspring were then backcrossed to this same testing stock and the Pax3Sp carrying In(1)1Rk mice were either sibling mated or backcrossed to this testing stock to look for recessive lethal mutations co-segregating with the Chromosome 1 markers or the Chromosome 7 markers. Lethal number 5 was found in animal number 959 and the recombination data indicated that the mutation is either within the In(1)1Rk inversion segment or very close to it. The embryos frozen were produced by breeding Pax3Sp Mlphln/+ Mlphln OR +Mlphln/+ Mlphln females with Pax3Sp Mlphln+/+ + l1Rk5 males. (Roderick TH, 1983; Giometti et al., 1990.)
|Allele Name||lethal, Chr 1, Roderick 5|
|Allele Type||Chemically induced (other)|
|Gene Symbol and Name||l1Rk5, lethal, Chr 1, Roderick 5|
|Strain of Origin||C57BL/6J x DBA/2J|
|Molecular Note||This mutation was induced by exposure of males with an inversion on chromosome 1 to 0.3mg/kg of triethylene melamine.|