B6(C)-H2-Ab1^bm12/KhEgJ

Stock number: 001162
Product name: bm12
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

This variant MHC class II allele, H2-Ab1^bm12, is associated with increased resistance to experimental autoimmune myasthenia gravis (EAMG). Mice congenic for H2 alleles are widely used in immunologic research and often vary in immune response and pathogen susceptibility from the recipient strain.

Detailed description

H2-Ab1^bm12 differs from H2-Ab1^b by 3 nucleotides resulting in amino acid substitutions in three codons in the beta1 exon. Surface expression of MHC class II A is reduced as is the amount of invariant chain co-precipitated with the alpha and beta chains. This variant MHC class II allele is associated with increased resistance to experimental autoimmune myasthenia gravis (EAMG). Homozygous mice exhibit lower IFNG and IL10 release and decreased calcium mobilization in response to immunization with acetylcholine receptor and its dominant peptide alpha146-162. Like other mice with the H2^b haplotype, this strain is MHC class II IE^b; the b allele is considered a null allele. Mice congenic for H2 alleles are widely used in immunologic research and often vary in immune response and pathogen susceptibility from the recipient strain.

Development

The H2-Ab1^bm12 allele originated as a spontaneous mutation in a C57BL/6Kh x BALB/cKh F1 female, and was shown to have occurred in the C57BL/6Kh H2^b haplotype. The female was crossed to the C57BL/6Kh background for 10 generations. This variant MHC class II allele differs from H2-Ab1^b by 3 nucleotides resulting in amino acid substitutions in three codons in the beta1 exon (Ile67Phe, Arg70Gln, Thr71Lys). This strain was transferred from Dr. Kohn (Harvard) to The Jackson Laboratory by Dr. Egorov.

Allele

Symbol: H2-Ab1^bm12
Name: b haplotype mutation 12
MGI accession ID: MGI:3586447
Generation method: Spontaneous
Marker symbol: H2-Ab1
Marker name: histocompatibility 2, class II antigen A, beta 1
Chromosome: 17
Strain of origin: (C57BL/6 x BALB/c)F1
Molecular note: Three non-consecutive nucleotide changes occurred, resulting in three amino acid substitutions in the beta1 exon. The amino acid changes consist of codon 67 (isoleucine to phenylalanine), codon 70 (arginine to glutamine) and codon 71 (threonine to lysine). These changes were likely the product of gene conversion of H2-Ab1^b with sequence from H2-Eb^b.
General note: Genbank ID for this allele: M54876