This strain is homozygous for nonagouti and segregating for Hpt.
This inbred strain carries the Tal1Hpt (hair patches) spontaneous mutation.
This strain was developed by Priscilla Lane in 1988 from a spontaneous mutation then named hair patches (Hpt, Mouse News Letter 65:29) which arose in a segregating hybrid (C57BL/6JxC3HeB/FeJLe-a/a) background at N3 in 1979.The Hpt mutation was backcrossed three times to this hybrid, then maintained as a segregating inbred strain. It was cryopreserved in 1989 with heterozygote by wild-type matings.
|Gene Symbol and Name||a, nonagouti|
|Strain of Origin||old mutant of the mouse fancy|
|General Note||Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039|
|Molecular Note||Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for at-2Gso and Aw-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type.|
|Allele Name||hair patches|
|Gene Symbol and Name||Tal1, T cell acute lymphocytic leukemia 1|
|Strain of Origin||(C57BL/6J x C3FeLe.B6-a/a)F1|
|Molecular Note||An endogenous retroviral class II intracisternal A particle (IAP) inserted into intron 4. Quantitative real time PCR revealed differential expression of one gene in the interval, T-cell acute lymphocytic leukemia 1 (Tal1), which was highly expressed in kidney and skin.|