These mice carry a spontaneous remutation at the Kit locus characterized by an irregular white band in the trunk region, a white head spot and a very mild anemia in homozygotes.Read More +
In mice carrying the KitW-57J remutation the temporal and spatial patterns of Kit expression are dysregulated during embryogenesis and in adult animals. The KitW-57J allele leads to an intrinsic pigmentation defect by downregulating developmental Kit expression in trunk melanoblasts, but not melanoblasts around the otic vesicle. The mutation affects transcriptional initiation of the Kit gene and is associated with a 80 kb deletion 5' of the Kit-coding region. The spontaneous mutation gives rise to an irregular white band in the trunk region, a white head spot and a very mild anemia in homozygotes. Heterozygous animals have a white spot in the ventral trunk region. The KitW-57J allele does not affect fertility.
The KitW-57J remutation occurred in 1998 on C58/J (Stock No. 000669), and was cryopreserved in 1988 by mating homozygous males to C57BL/6J females.
|Allele Name||dominant spotting 57 Jackson|
|Gene Symbol and Name||Kit, KIT proto-oncogene receptor tyrosine kinase|
|Strain of Origin||C58/J|
|Molecular Note||Analysis using pulsed-gel electrophoresis revealed that this allele comprises an 80 kb deletion located at the 5' end of the Kit-coding sequence.|