Overview

Also Known As:bradypneic

This inbred mutant strain carries the the extreme nonagouti (a^e) and white bellied agouti Jackson (A^w-J) coat color mutations along with the bradypneic (bd) spontaneous mutation.

Genetic overview

Genetic Background	Generation

a^e

Allele Type	Gene Symbol	Gene Name
Radiation induced	a	nonagouti

A^w-J

Allele Type	Gene Symbol	Gene Name
Spontaneous	a	nonagouti

Allele Type	Gene Symbol	Gene Name
Spontaneous	bd	bradypneic

Research Applications

Dermatology Research
Internal/Organ Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Important Note

This strain is segregating for recessive a^e and dominant A^w-J.

Detailed Description

Homozygous bd/bd mice show variable weight retardation beginning at 3 to 5 days. Severely affected mice die within the first two weeks; less severely affected mice may survive to weaning or to adulthood and may be fertile. Homozygotes tested at 3 weeks or older breathe at half the normal rate, but breathing is deeper and 02 consumption per unit of body surface is normal. Blood pH and blood C02 capacity are normal, and no obstruction has been found in the nasal passages, larynx, trachea, or bronchi. The lungs are somewhat emphysematous and the right atrium is enlarged. There is dilation of some of the distal tubules of the kidney, and large amounts of gas in the stomach and intestines. The cause of the breathing defect is not known, but the breathing defect may be responsible for the emphysema, intestinal gas, small size, and early death. (J:65322 Green MC, et al., bd - bradypneic. Mouse News Lett. 1977;56:40)

Development

Bradypneic (bd) was found in December 1969 in the 21st generation of inbreeding of a strain segregating for A^w-J and a^e/a^e. Embryos produced by mating either A^w-J/a^e bd/bd or a^e/a^e bd/bd males to AEJ/GnLeJ (Stock No. 000199) females were cryopreserved in 1983.

Control Suggestions

Untyped from the colony

Additional Information

Considerations for Choosing Controls

Genetics

a^e

Allele Symbol: a^e

Allele Name	extreme nonagouti
Allele Type	Radiation induced
Allele Synonym(s)
Gene Symbol and Name	a, nonagouti
Gene Synonym(s)
Strain of Origin	(P x S)F1
Chromosome	2
General Note	The a^e mutation was found among descendants of an irradiated mouse. a^e is recessive to all other alleles except a^l.

A^w-J

Allele Symbol: A^w-J

Allele Name	white bellied agouti Jackson
Allele Type	Spontaneous
Allele Synonym(s)	A^WJ
Gene Symbol and Name	a, nonagouti
Gene Synonym(s)
Strain of Origin	C57BL/6J
Chromosome	2

bd

Allele Symbol: bd

Allele Name	bradypneic
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	bd, bradypneic
Gene Synonym(s)
Strain of Origin	AEJ
Chromosome	12

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: a^e related

Dermatology Research
- Color and White Spotting Defects

Genotype: bd related

Internal/Organ Research
- Gastrointestinal Defects
- Lung Defects

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: bd/bd
AEJ/Gn-bd/J

cardiovascular system phenotype

enlarged heart right atrium
- at 10 days of age, the right atrium is enlarged, probably as a result of emphysematous changes

growth/size/body region phenotype

abnormal soft palate morphology
- at 10 days of age, the posterior portion of the tongue is depressed and the soft palate is elevated making a small cavity not found in control mice

decreased body size
- homozygotes appear runted starting at ~5 days after birth
- occasionally, less severely runted mutants survive to adulthood; these often attain a nearly normal size and are usually fertile

meteorism
- at 4-15 days of age, all bradypneic mutants contain significant amounts of gas in the small and large intestines
- gas is not always present in the stomach of bradypneic mutant mice at 4-15 days of age; however, when present, it is usually found in large amounts
- the stomach and intestines are filled with large amounts of gas

decreased body weight
- by 7-10 days after birth, most surviving homozygotes weigh only ~50% or less than wild-type littermates

abnormal tongue morphology
- at 10 days of age, the posterior portion of the tongue is depressed and the soft palate is elevated making a small cavity not found in control mice

cachexia
- homozygotes that are either moribund or dead at preweaning stages usually display severe emaciation
- however, occasionally, presumed mutant mice appear to have died at 1 to 2 weeks of age in an apparently healthy condition

slow postnatal weight gain
- homozygotes display variable weight retardation starting at 3-5 days after birth

mortality/aging

postnatal lethality, incomplete penetrance
- less severely affected mutants may survive to weaning or to adulthood and may be fertile
- only 25% of mutants survive to weaning; infrequently a mouse survives to breeding age and is fertile
- severely affected homozygotes die within the first 2 weeks after birth

renal/urinary system phenotype

dilated renal tubules
- at 4-29 days of age, all mutant mice exhibit moderate or severe dilation of many of the distal convoluted renal tubules
- however, all proximal tubules, collecting tubules, and glomeruli appear normal
- some of the distal renal tubules appear dilated

craniofacial phenotype

abnormal soft palate morphology
- at 10 days of age, the posterior portion of the tongue is depressed and the soft palate is elevated making a small cavity not found in control mice

abnormal tongue morphology
- at 10 days of age, the posterior portion of the tongue is depressed and the soft palate is elevated making a small cavity not found in control mice

respiratory system phenotype

decreased pulmonary respiratory rate
- polygraph recordings indicate that mutant mice breathe more deeply than control mice; however, sniffing rates are within the normal range
- at 3 weeks of age or later, surviving homozygotes breathe at half the normal rate (bradypneic) but breathing is deeper
- however, oxygen consumption per unit of body surface, blood pH, and blood CO₂ capacity are normal
- starting at 10 days of age, all bradypneic homozygotes display a ~50% reduction in breathing frequency relative to heterozygous controls (measured as 130 vs 264 breaths/min, respectively, at 4 weeks of age)
- no obstruction is detected in the nasal passages, larynx, trachea or bronchi

emphysema
- by 23 days of age, mutant lungs display significant flattening of the alveoli and expansion of the alveolar ducts relative to control lungs
- mutant lungs appear somewhat emphysematous

digestive/alimentary phenotype

abnormal soft palate morphology
- at 10 days of age, the posterior portion of the tongue is depressed and the soft palate is elevated making a small cavity not found in control mice

meteorism
- at 4-15 days of age, all bradypneic mutants contain significant amounts of gas in the small and large intestines
- gas is not always present in the stomach of bradypneic mutant mice at 4-15 days of age; however, when present, it is usually found in large amounts
- the stomach and intestines are filled with large amounts of gas

abnormal tongue morphology
- at 10 days of age, the posterior portion of the tongue is depressed and the soft palate is elevated making a small cavity not found in control mice

References

Additional - a^e related

Additional - A^w-J related

Additional - bd related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:A A A Alternate2
- Genotyping resources and troubleshooting
Citation
When using the bradypneic mouse strain in a publication, please cite the originating article(s) and include JAX stock #000202 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Compound Heterozygous for allele A<w>and allele a<e> or Homozygous for a<e> and Heterozygous for +/bd

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:bradypneic

Genetic overview

Research Applications

Base Price

Important Note

Detailed Description

Development

Control Suggestions

Additional Information

ae

Allele Symbol: ae

Aw-J

Allele Symbol: Aw-J

bd

Allele Symbol: bd

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: ae related

Genotype: bd related

Mammalian Phenotype Terms by Genotype

Genotype: bd/bdAEJ/Gn-bd/J

cardiovascular system phenotype

growth/size/body region phenotype

mortality/aging

renal/urinary system phenotype

craniofacial phenotype

respiratory system phenotype

digestive/alimentary phenotype

References

Additional - ae related

Additional - Aw-J related

Additional - bd related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

a^e

Allele Symbol: a^e

A^w-J

Allele Symbol: A^w-J

Genotype: a^e related

Genotype: bd/bd
AEJ/Gn-bd/J

Additional - a^e related

Additional - A^w-J related