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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
9369 | CTG GAG CTC TAC AGT GAT GA | Forward | ||||
9370 | CAG TTA CCA ATC AAC ACA TCA C | Reverse | ||||
9371 | AGC CCT ACA GCC ACA TGA TA | Transgene Forward | Sry | |||
9372 | GTC TTG CCT GTA TGT GAT GG | Transgene Reverse | Sry | |||
oIMR8744 | CAA ATG TTG CTT GTC TGG TG | Internal Positive Control | ||||
oIMR8745 | GTC AGT CGA GTG CAC AGT TT | Internal Positive Control |
Component | Final Concentration |
---|---|
ddH2O | |
KAPA Rxn Buffer Contains 1.5 mM Mg2+ final concentration. | 1.00 |
MgCl2 | 0.00 |
dNTP | 0.20 |
9369 | 1.00 |
9370 | 1.00 |
9371 | 1.00 |
9372 | 1.00 |
oIMR8744 | 0.10 |
oIMR8745 | 0.10 |
DNA Loading Dye | 0.69 |
KAPATaq DNA Polymera No proofreading function. 1 error per 40000 nucleotides. Initial denature 2 min. | 0.03 |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 60.0 | -- | |
4 | 72.0 | -- | |
5 | -- | Go to step 2-4, 35 times | |
6 | 72.0 | -- | |
7 | 10.0 | -- | hold |
Stock Number | Strain Name |
---|---|
010905 | B6.Cg-Tg(Sry)2Ei Srydl1Rlb T(XTmsb4x-Hccs;Y)1Dto/ArnoJ |
039108 | B6.Cg-Tg(Sry)2Ei Srydl1Rlb/JtuJ |
002063 | C57BL/6JEi-Tg(Sry)1Ei Chr YAKR/J/EiJ |
002708 | C57BL/6JEi-Tg(Sry)2Ei Chr YAKR/J/EiJ |
002837 | C57BL/6JEi-Tg(Sry)3Ei Chr YAKR/J/EiJ |
002183 | C57BL/6JEi-Tg(Sry)4Ei Chr YPOS/EiJ |
027001 | QSi5/IanmTaftJ |
7 strains use this protocol |